Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo.
Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo.
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Tenuigenin 促进体内外骨间充质干细胞的成骨分化
DOI：
10.1007/s00441-016-2528-1
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发表时间：
2017-02
影响因子：
3.6
通讯作者：
Zhang ZM
中科院分区：
文献类型：
--
作者：
Jiang HJ;Tian XG;Huang SB;Chen GR;Huang MJ;Chen YH;Yan B;Li SF;Tang JJ;Zhao HY;Wang L;Zhang ZM
骨质疏松症是一种以骨密度降低和骨质量微结构恶化为特征的全身性骨骼疾病，是一个全球性且日益严重的公共卫生问题。最近的研究表明，Tenuigin(TEN)是一类具有抗吸收等多种生物活性的天然化合物，对绝经后的骨丢失具有保护作用。本研究旨在研究TEN在体内外对骨髓间充质干细胞(BMSCs)的成骨作用。对成骨条件下培养2周的C57/BL6小鼠原代股骨BMSCs进行碱性磷酸酶染色、茜素红染色及成骨标记物：矮小相关转录因子2、Osterix、骨钙素、I型胶原α1、β-连环蛋白和糖原合成酶β表达的检测。采用去卵巢(OVX)小鼠模型，观察Ten治疗3个月的体内效应。体外和体内实验发现，碱性磷酸酶活性、矿化结节和成骨标志物表达增加，Wnt/β-catenin信号转导增强。OVX+TEN组小鼠的骨小梁厚度、骨小梁数目和骨密度等骨参数均高于对照组。我们的结果提示TEN有治疗绝经后骨质疏松症的潜力。
Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of bone quality, is a global and increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), a class of native compounds with numerous biological activities such as anti-resorptive properties, exerts protective effects against postmenopausal bone loss. The present study aims to investigate the osteogenic effects of TEN on bone mesenchymal stem cells (BMSCs) in vitro and in vivo. Alkaline phosphatase (ALP) activity/staining, Alizarin red staining and the expression of osteogenic markers, including runt-related transcription factor 2, osterix, osteocalcin, collagen Iα1, β-catenin and glycogen synthase kinase-3β were investigated in primary femoral BMSCs from C57/BL6 mice cultured under osteogenic conditions for 2 weeks to examine the effects of TEN. An ovariectomized (OVX) mouse model was used to investigate the effect of TEN treatment for 3 months in vivo. We found that ALP activity, mineralized nodules and the expression of osteogenic markers were increased and WNT/β-catenin signaling was enhanced in vitro and in vivo. Bone parameters, including trabecular thickness, trabecular number and bone mineral density were higher in the OVX+TEN group than in control OVX mice. Our results suggest the therapeutic potential of TEN for the treatment of patients with postmenopausal osteoporosis.