Horseradish peroxidase catalyzed degradation of industrially important dyes.
Horseradish peroxidase catalyzed degradation of industrially important dyes.
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DOI：
10.1002/1097-0290(20010305)72:5
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发表时间：
2001-03
影响因子：
3.8
通讯作者：
A. Bhunia;Susheel Durani;P. Wangikar
中科院分区：
文献类型：
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作者：
A. Bhunia;Susheel Durani;P. Wangikar
辣根过氧化物酶（HRP）是已知的降解某些难降解的有机化合物，如苯酚和取代苯酚。在这里，我们第一次证明了HRP在降解和沉淀工业上重要的偶氮染料方面是有效的。对于雷马唑蓝，酶的活性被发现是远远优于在pH 2.5比在中性pH值。此外，雷马唑蓝作为一个强有力的竞争性抑制剂的HRP在中性pH值。辣根过氧化物酶显示了广泛的底物特异性对各种偶氮染料。测定了两种不同染料的动力学常数K（m）（app）和V（max）（app）。本研究除了对HRP降解染料的潜力进行系统分析外，还为开发商业染料作为酶抑制剂开辟了新的领域。John Wiley & Sons，Inc.
Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP to be effective in degrading and precipitating industrially important azo dyes. For Remazol blue, the enzyme activity was found to be far better at pH 2.5 than at neutral pH. In addition, Remazol blue acts as a strong competitive inhibitor of HRP at neutral pH. Horseradish peroxidase shows broad substrate specificity toward a variety of azo dyes. Kinetic constants (K(m)(app) and V(max)(app)) for two different dyes have been determined. In addition to providing a systematic analysis of the potential of HRP in degradation of dyes, this study opens up a new area on exploration of commercial dyes as inhibitors of enzymes. 2001 John Wiley & Sons, Inc.