The histochemistry of mucins in certain primate salivary glands.
The histochemistry of mucins in certain primate salivary glands.
复制标题
某些灵长类唾液腺中粘蛋白的组织化学。
作者：
T. J. Leppi;S. Spicer
松鼠、恒河猴和人的颌下腺和舌下腺含有组织化学证明的中性粘蛋白、唾液粘蛋白和磺粘蛋白。根据不同染色程序的结果，以及先前唾液酸酶消化、高碘酸氧化和某些化学修饰对几种染色程序的影响，每种细胞类型内的粘液基质类型都有差异。具体地说，含唾液酸的粘液糖均匀存在于两种猕猴颌下腺的粘液细胞和恒河猴舌下腺的后段。S35O4放射自显影证实，磺粘蛋白是松鼠猴舌下腺的主要分泌物，也存在于恒河猴舌下腺的前部。后者与少量唾液粘蛋白混合。这两种粘蛋白中的一种或另一种或两种粘蛋白的混合物以大致相等的比例存在于人颌下腺的粘液腺泡中。人类颌下腺中的浆液性腺泡形成颗粒分泌物，与乙醛品红有很强的亲和力，可能是由于一种硫酸盐化的粘膜基质。腺体中其他的，也许是一些相同的浆液性腺泡，在唾液酸酶之后的高碘酸盐-对二胺程序中可能产生含有唾液酸的颗粒状分泌物，变成己糖或脱氧己糖。人舌下腺粘液腺泡中的主要分泌成分是磺化粘蛋白。然而，舌下腺中的一些粘液腺泡在不同或相同的细胞中产生唾液和硫粘蛋白的混合物。人类舌下腺的浆膜粘液性半月体和颌下腺一样产生富含唾液酸的颗粒状分泌物。
Submandibular and sublingual salivary glands of squirrel and rhesus monkeys and man contain histochemically demonstrable neutral mucosubstances, sialomucins and sulfomucins. Judged by results obtained with a variety of staining procedures and by the effects of prior sialidase digestion, periodate oxidation and certain chemical modifications on the several staining procedures, differences are revealed in the types of mucosubstances within each cell type. Specifically, sialic acid-containing mucosaccharides are present uniformly in the mucous cells of the submandibular glands of both monkeys and in a posterior segment of the sublingual gland of the rhesus monkey. Sulfomucin is the predominant secretion of the sublingual gland of the squirrel monkey and is also found in the anterior portion of the sublingual of the rhesus monkey as verified by S35O4 = autoradiography. This latter sulfomucin is admixed with a small amount of sialomucin. One or the other or mixtures of these two mucins in about equal proportions are present in the mucous acini of human submandibular glands. Seromucous acini in the human submandibular gland form a granular secretion with strong affinity for aldehyde fuchsin possibly attributable to a sulfated mucosubstance. Other and perhaps some of the same seromucous acini in this gland produce a granular secretion containing sialic acid in probable proximity, as shown by the periodate-para diamine procedure after sialidase, to a hexose or deoxyhexose. A sulfomucin forms the major secretory component in the mucous acini of human sublingual glands. However, some mucous acini in this sublingual gland produce a mixture of sialo- and sulfomucins either in different or the same cells. Seromucous demilunes or acini in the human sublingual gland produce a granular secretion rich in sialic acid like that in the submandibular gland.