The effects of prolonged opioidergic blockade on LH pulsatile secretion during the menstrual cycle.
The effects of prolonged opioidergic blockade on LH pulsatile secretion during the menstrual cycle.
复制标题
长期阿片类药物阻断对月经周期 LH 脉动分泌的影响。
DOI：
10.1007/bf03349974
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发表时间：
1989
影响因子：
5.4
通讯作者：
Yen,SS
中科院分区：
文献类型：
--
作者：
Rossmanith,WG;Wirth,U;Sterzik,K;Yen,SS
虽然大量证据表明内源性阿片肽（EOP）在GnRH-LH分泌的神经内分泌调节中起着关键作用，但长期阿片能阻滞对月经周期中LH脉动活性的影响尚未得到彻底研究。因此，在阿片受体拮抗剂纳洛酮实施阿片能阻滞之前和期间连续两天研究了10名卵泡早期（EFP，第3天和第4天）、10名卵泡晚期（LFP，第9天至第13天）和7名黄体中期（MLP，LH峰后第6天至第8天）的女性。在生理盐水（150 mmol/l，50 ml/h）或纳洛酮（30μg/kg/h）输注期间，以15 min间隔采集血样，持续8 h。此外，在其他6名女性中（EFP、LFP和MLP中各2名）连续24小时输注生理盐水或纳洛酮（30 μg/kg/h）。通过聚类脉冲算法分析LH激素系列的显著脉冲。虽然8小时纳洛酮输注未改变EFP中的任何LH脉冲特征（频率、幅度、横向平均值、持续时间），但它们显著升高了LFP中的LH脉冲频率、脉冲幅度和横向平均水平（p< 0.05）。在MLP中，LH脉冲幅度显著增加（p< 0.05），但脉冲频率和横向平均水平保持不变。虽然24小时纳洛酮输注未改变EFP中的任何脉冲特征，但它们引起LFP中LH脉冲活动的稳健增加，包括LH脉冲幅度和横向平均水平的逐渐升高。LH脉冲频率保持不变。尽管24小时纳洛酮输注增加了MLP中的LH脉冲频率，但它们倾向于降低LH脉冲幅度，因此LH横向平均水平保持不变。在周期的任一阶段，纳洛酮输注期间的LH脉冲持续时间与对照条件无差异。这些观察结果证实，在月经周期中，长期阿片受体阻滞剂对LH分泌的影响各不相同，取决于当时的卵巢类固醇环境。我们的研究结果进一步表明，在月经周期中，长时间的阿片类药物阻滞对LH脉冲频率、脉冲幅度和横向平均水平产生不同的影响。因此，可以推断，内源性阿片肽在月经周期中的LH脉动活动的调节不同程度地参与。
Although considerable evidence points towards a pivotal role for the endogenous opioid peptides (EOP) in the neuroendocrine regulation of GnRH-LH secretion, the effects of prolonged opioidergic blockade on LH pulsatile activity during the menstrual cycle have not been thoroughly investigated. Accordingly, 10 women in the early follicular phase (EFP, days 3 and 4), 10 women in the late follicular phase (LFP, days 9 to 13) and 7 women in the midluteal phase (MLP, days 6 to 8 after LH surge) were studied on two consecutive days before and during opioidergic blockade imposed by an opiate receptor antagonist, naloxone. Blood samples were obtained at 15 min intervals for 8 h during saline (150 mmol/l at 50 ml/h) or naloxone (30μg/kg/h) infusions. Furthermore, sequential 24-h infusions of saline or naloxone (30 μg/kg/h) were performed in 6 other women (two each in the EFP, LFP, and MLP). LH hormone series were analyzed for significant pulses by the Cluster pulse algorithm. While 8-h naloxone infusions did not change any of the LH pulse characteristics (frequency, amplitude, transverse mean, duration) in the EFP, they elevated significantly (p< 0.05) the LH pulse frequencies, pulse amplitudes and transverse mean levels in the LFP. In the MLP, the LH pulse amplitudes were significantly (p< 0.05) increased, but pulse frequencies and transverse mean levels remained unchanged. While the 24-h naloxone infusions did not alter any of the pulse characteristics in the EFP, they elicited a robust increase in LH pulsatile activity in the LFP, composed of a progressive rise in LH pulse amplitudes and transverse mean levels. The LH pulse frequencies remained unchanged. Although the 24-h naloxone infusions increased the LH pulse frequency in the MLP, they tended to decrease the LH pulse amplitude, so that the LH transverse mean levels remained unaltered. The LH pulse durations during naloxone infusions were not different from the control conditions in either phase of the cycle. These observations confirm that the effects of prolonged opioidergic blockade on LH secretion vary during the menstrual cycle, dependent on the prevailing ovarian steroid milieu. Our findings further demonstrate that a prolonged opiate blockade exerts different on LH pulse frequency, pulse amplitude and transverse mean levels during the menstrual cycle. Thus, it may be inferred that endogenous opioid peptides are variously involved in the regulation of LH pulsatile activity during the menstrual cycle.