Evaluation of the dried blood spot filter paper technology and five testing strategies of HIV-1 and HIV-2 infections in West Africa
Evaluation of the dried blood spot filter paper technology and five testing strategies of HIV-1 and HIV-2 infections in West Africa
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DOI：
10.1080/00365540600801645
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发表时间：
2006-11-01
影响因子：
--
通讯作者：
Whittle, Hilton
中科院分区：
文献类型：
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作者：
Sarge-Njie, Ramu;Van der Loeff, Maarten Schim;Whittle, Hilton
需要采取简单有力的办法来监测非洲艾滋病毒的流行和发病率。本研究的目的是评估干血斑（DBS）作为血清或血浆替代品用于哨兵监测的使用。配对DBS和血液样本来自200名参加西非泌尿生殖医学诊所的患者。诊断的金标准是基于使用血清的3种酶联免疫吸附试验（ELISA）的组合。采用ELISA、明胶颗粒试验（GPA）和Pepti-Lav 1-2在5种不同的检测策略中检测干血斑洗脱液中HIV抗体的存在。对所有200份洗脱液进行单独检测，并对各含5份洗脱液的合并液进行检测。检测策略的敏感性范围为95.0%（83.1-99.4%）至100%，特异性范围为97.5%（93.7-99.3%）至100%。在5个样本池中进行检测不影响灵敏度。干血迹很容易处理。检测试剂盒和实验室消耗品的成本在492磅和1037磅（未合并策略）和163磅和421磅（合并策略）之间变化。本研究中使用的单特异性ELISA已不再生产;需要测试目前可用的分化测定法。DBS被推荐用于非洲的哨点监测。
Simple robust approaches are needed to monitor the prevalence and incidence of HIV in Africa. The aim of this study was to evaluate the use of dried blood spot (DBS) as an alternative to serum or plasma for sentinel surveillance. Paired DBS and blood samples were obtained from 200 patients attending a genito-urinary medicine clinic in West Africa. The gold standard of diagnosis was based on the combination of 3 enzyme-linked immunosorbent assays (ELISA) using serum. The presence of HIV antibodies in eluates of dried blood spots was detected by ELISA, Gelatin Particle Assay (GPA) and Pepti-Lav 1-2 in 5 different testing strategies. All 200 eluates were tested individually, and in addition pools of 5 eluates each were tested. The sensitivity of the testing strategies ranged from 95.0% (83.1-99.4%) to 100% and the specificity from 97.5% (93.7-99.3%) to 100%. Testing in pools of 5 did not affect sensitivity. Dried blood spots were easy to work with. Test kit and laboratory consumable costs varied between 492 pound and 1037 pound (unpooled strategies) and 163 pound and 421 pound (pooled). The monospecific ELISAs used in this study are no longer in production; currently available differentiating assays need to be tested. DBS are recommended for sentinel surveillance in Africa.