The Aspergillus fumigatus toxin fumagillin suppresses the immune response of Galleria mellonella larvae by inhibiting the action of haemocytes
The Aspergillus fumigatus toxin fumagillin suppresses the immune response of Galleria mellonella larvae by inhibiting the action of haemocytes
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DOI：
10.1099/mic.0.043786-0
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发表时间：
2011-05-01
期刊：
影响因子：
2.8
通讯作者：
Kavanagh, Kevin
中科院分区：
文献类型：
--
作者：
Fallon, John P.;Reeves, Emer P.;Kavanagh, Kevin
大蜡螟幼虫被广泛用于评价微生物毒力和评价抗菌药物的体内效力。这项工作的目的是检查烟曲霉毒素，烟曲霉素，抑制幼虫的免疫反应的能力。给幼虫施用烟曲霉素增加了它们对随后感染A.烟曲霉分生孢子（P=0.0052）。结果表明，2 μ g烟曲霉素m1（-1）的剂量降低了昆虫免疫细胞（血细胞）杀死白色念珠菌调理细胞（P=0.039）和吞噬A的能力。烟曲霉分生孢子（P=0.016）。烟曲霉素减少血细胞的氧摄取，并减少与p47（Phox）同源的p47蛋白的易位，p47蛋白是形成超氧化物产生所需的功能性NADPH氧化酶复合物所必需的蛋白质。此外，毒素处理的血细胞表现出降低的脱粒水平，这是通过对抗髓过氧化物酶抗体显示反应性的蛋白质的释放来测量的（P
Larvae of Galleria mellonella are widely used to evaluate microbial virulence and to assess the in vivo efficacy of antimicrobial agents. The aim of this work was to examine the ability of an Aspergillus fumigatus toxin, fumagillin, to suppress the immune response of larvae. Administration of fumagillin to larvae increased their susceptibility to subsequent infection with A. fumigatus conidia (P=0.0052). It was demonstrated that a dose of 2 mu g fumagillin m1(-1) reduced the ability of insect immune cells (haemocytes) to kill opsonized cells of Candida albicans (P=0.039) and to phagocytose A. fumigatus conidia (P=0.016). Fumagillin reduced the oxygen uptake of haemocytes and decreased the translocation of a p47 protein which is homologous to p47(Phox), a protein essential for the formation of a functional NADPH oxidase complex required for superoxide production. In addition, toxin-treated haemocytes showed reduced levels of degranulation as measured by the release of a protein showing reactivity to an anti-myeloperoxidase antibody (P