Identification of a new endothelial cell growth factor receptor tyrosine kinase.
Identification of a new endothelial cell growth factor receptor tyrosine kinase.
复制标题
DOI：
--
复制
发表时间：
1991-09
期刊：
影响因子：
8
通讯作者：
B. I. Terman;M. E. Carrion;E. Kovács;Rasmussen Ba;Eddy Rl;T. Shows
中科院分区：
文献类型：
--
作者：
B. I. Terman;M. E. Carrion;E. Kovács;Rasmussen Ba;Eddy Rl;T. Shows
从人内皮细胞cDNA文库中克隆了一个新的生长因子受体酪氨酸激酶（RTK）基因。使用聚合酶链反应（PCR）和与插入结构域侧保守的酪氨酸激酶结构域互补的退化寡核苷酸引物鉴定了该基因，这是已知III型rtk的特征[例如血小板衍生生长因子受体（PDGF-R）、集落刺激-1受体（CSF-1-R）、成纤维细胞生长因子受体（FGF-R）和ckit]。然后用PCR的DNA产物作为探针，从cDNA文库中分离编码受体的较大DNA片段。预测的氨基酸序列包含典型的III型受体酪氨酸激酶的多个特征（即atp结合位点，跨膜区域，分裂酪氨酸激酶区域）。KDR基因以7.0 kb的转录本表达，定位于人类4号染色体。
A new growth factor receptor tyrosine kinase (RTK) gene (designated KDR) has been cloned from a human endothelial cell cDNA library. The gene was identified using the polymerase chain reaction (PCR) and degenerate oligonucleotide primers complementary to conserved tyrosine kinase domains that flank the insert domain, characteristic of known type III RTKs [e.g. platelet-derived growth factor receptor (PDGF-R), colony-stimulating-1 receptor (CSF-1-R), fibroblast growth factor receptor (FGF-R) and ckit]. The DNA product from PCR was then used as a probe to isolate larger DNA segments encoding the receptor from the cDNA library. The predicted amino acid sequence contained multiple characteristics (i.e. an ATP-binding site, a membrane-spanning region, split tyrosine kinase regions) typical of a type III receptor tyrosine kinase. The KDR gene is expressed as a 7.0 kb transcript, and is localized to human chromosome 4.