Wheat methionine sulfoxide reductase genes and their response toabiotic stress
Wheat methionine sulfoxide reductase genes and their response toabiotic stress
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小麦蛋氨酸亚砜还原酶基因及其对非生物胁迫的响应
DOI：
10.1007/s11032-016-0597-1
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
夏光敏
中科院分区：
文献类型：
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作者：
丁鹏程;高艳坤;朱建堂;陈凡国;夏光敏
小麦品种陕荣3号（cv. SR3）比其祖先品种济南177（cv. JN177）。当品种受到胁迫时，已知编码甲硫氨酸亚砜还原酶（MSRs）的许多基因在SR3中上调。在这里，一组12个全长小麦MSR（TaMSR）cDNA已被分离的cv。SR 3.这些基因在不同发育阶段的小麦根、茎和叶中转录。盐和干旱诱导的突变体在其启动子区含有已知的胁迫响应元件。利用基因芯片技术对盐和干旱诱导的4个MSR基因在拟南芥中的组成型表达进行了分析，结果表明：TaMSRA 2的表达产物提高了植物对NaCl、甲基紫精和脱落酸的耐受性，TaMSRA 5的表达产物提高了植物的耐盐性。TaMSRB 1.1提高了对盐、MV和H_2O_2的耐性，而TaMSRB 5.1提高了对盐和甘露醇的耐性。的影响，在A。TaMSRB 1.1的作用主要是抑制活性氧的积累和增加胞内可溶性糖的含量。
The wheat cultivar Shanrong no. 3 (cv. SR3) tolerates both salinity and drought stress more effectively than does its progenitor cultivar Jinan 177 (cv. JN177). When the cultivars are subjected to stress, a number of genes encoding methionine sulfoxide reductase (MSRs) are known to be upregulated in SR3. Here, a set of 12 full lengthTriticum aestivum MSR(TaMSR) cDNAs have been isolated from cv. SR3. The genes were transcribed in the wheat root, stem, and leaf in plants sampled at various developmental stages. Those induced by salinity and drought harbored known stress-responsiveciselements in their promoter region. The constitutive expression inArabidopsis thalianaof four MSRs which were induced by salt and drought in microarray assay showed that the product of one (TaMSRA2) heightened the plant’s tolerance to NaCl, methylviologen (MV), and abscisic acid, that of the second (TaMSRA5) enhanced salinity tolerance, that of the third (TaMSRB1.1) increased tolerance to salinity, MV and H2O2, and that of the fourth (TaMSRB5.1) increased tolerance to both salinity and mannitol. The effect of the presence inA. thalianaof TaMSRB1.1 was to suppress the accumulation of reactive oxygen species and to increase the intracellular content of soluble sugars.