Multiscale 3D Virtual Dissections of 100-Million- Year- Old Flowers Using X- Ray Synchrotron Microand Nanotomography
Multiscale 3D Virtual Dissections of 100-Million- Year- Old Flowers Using X- Ray Synchrotron Microand Nanotomography
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DOI：
10.1017/s1431927613014025
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Tafforeau, Paul
中科院分区：
文献类型：
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作者：
Moreau, Jean-David;Cloetens, Peter;Tafforeau, Paul
采用相衬 X 射线微断层扫描和纳米断层扫描相结合的多尺度方法，对白垩纪化石花序进行成像，分辨率范围为 0.75 m 至 50 nm。广泛的比例视图提供了从花序片段的外部总体形态到原位花粉的最精细的外壁雕塑的三维重建。这种方法可以无损地获得通常在光学显微镜下或在扫描和透射电子显微镜下低放大倍率下观察到的大多数特征。与之前使用分辨率低至微米范围的化石和现存花朵的断层扫描研究相比，我们在局部断层扫描中使用边长为 50 nm 的体素。这种高水平的分辨率使得能够在不破坏或切片标本的情况下建立化石花卉的系统亲和力。
A multiscale approach combining phase-contrast X-ray micro- and nanotomography is applied for imaging a Cretaceous fossil inflorescence in the resolution range from 0.75 m to 50 nm. The wide range of scale views provides three-dimensional reconstructions from the external gross morphology of the inflorescence fragment to the finest exine sculptures of in situ pollen. This approach enables most of the characteristics usually observed under light microscopy, or with low magnification under scanning and transmission electron microscopy, to be obtained nondestructively. In contrast to previous tomography studies of fossil and extant flowers that used resolutions down to the micron range, we used voxels with a 50 nm side in local tomography scans. This high level of resolution enables systematic affinities of fossil flowers to be established without breaking or slicing specimens.