Extraction of essential oils of Ferulago angulata with microwave-assisted hydrodistillation
Extraction of essential oils of Ferulago angulata with microwave-assisted hydrodistillation
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DOI：
10.1016/j.indcrop.2019.05.015
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发表时间：
2019-10-01
影响因子：
5.9
通讯作者：
Asgharian, Parina
中科院分区：
文献类型：
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作者：
Mollaei, Saeed;Sedighi, Farzaneh;Asgharian, Parina
苦阿魏（Ferulago angulata）是伊朗重要的药用植物，具有多种药理作用，广泛应用于香料和医药工业。考虑到F.为了优化苦皮藤精油的微波辅助水蒸馏（MAHD）方法，本研究对苦皮藤精油进行了优化。以提高苦皮藤果实精油的产量和生物活性。通过响应面法（RSM）的中心组合设计（CCD），使用三个变量因素（水蒸馏时间，辐照功率和植物/液体比）的产率进行优化。结果表明，微波功率为980 W、料液比为2（g/100 mL）、微波时间为72 min时精油得率最高。结果表明，苦皮藤精油得率最高（6.50%），水蒸气蒸馏法（HD）精油得率最低（2.65%）。采用气相色谱-质谱（GC-MS）联用技术对MAHD和HD法提取的挥发油进行分析，结果表明其主要成分为柠檬烯、α-蒎烯、β-桉叶蓝烯、α-桉叶蓝烯和萜品油烯。抗氧化研究表明，MAHD精油比HD精油具有更高的抗氧化活性。此外，通过MAHD获得的精油显示出对MCF-7的有效抗增殖活性，IC 50为8.51 μ g/mL。本研究表明，MAHD是水蒸气蒸馏法提取红花挥发油的一种有效方法。与HD法相比，缩短了水蒸馏时间，降低了能耗，提高了生物活性。
Ferulago angulata (Apiaceae), an important medicinal plant of Iran, has been considered to have different therapeutical effects and are widely used in the perfume and pharmacological industries. Considering capability of F. angulata essential oils, the present study was conducted to optimized microwave-assisted hydrodistillation (MAHD) method for F. angulata fruit in order to improve the yield and biological activities of essential oils. Optimization of the yield was done through central composite design (CCD) of the response surface methodology (RSM) using three variable factors (hydrodistillation time, irradiation power and plant/liquid ratio). Based on the RSM results, the maximum essential oil yield was obtained with the microwave power of 980 W and plant/liquid ratio of 2 (g/100 mL) at 72 min. The results revealed that MAHD of F. angulata produced the highest essential oil yield (6.50%) as compared with essential oil prepared with hydrodistillation (HD) (2.65%). The essential oils extracted by MAHD and HD were analyzed by gas chromatography-mass spectroscopy (GC-MS) and indicated the main constituents of them were limonene, alpha-Pinene, beta- Phellandrene, alpha-Phellandrene, and Terpinolene. The antioxidant study revealed that MAHD essential oil had higher antioxidant activity than the HD essential oil. Furthermore, the essential oil obtained by MAHD demonstrated potent anti-proliferative activity against MCF-7 with IC50 8.51 mu g/mL. This research revealed that MAHD could be an effective method for hydrodistillation of essential oil in F. angulata in terms of shortening hydrodistillation time, reduction of energy consumption and increasing biological activities compared with the HD method.