Comparison of three-point bending test and peripheral quantitative computed tomography analysis in the evaluation of the strength of mouse femur and tibia
Comparison of three-point bending test and peripheral quantitative computed tomography analysis in the evaluation of the strength of mouse femur and tibia
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DOI：
10.1016/s8756-3282(98)00076-3
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发表时间：
1998-08-01
期刊：
影响因子：
4.1
通讯作者：
Tuukkanen, J
中科院分区：
文献类型：
--
作者：
Jämsä, T;Jalovaara, P;Tuukkanen, J
采用外周定量计算机断层扫描（pQCT）测量了10只雄性小鼠（体重32.8 +/- 4.0 g）的股骨和胫骨的体积皮质骨矿物质密度（VCtBMD）、中轴皮质横截面面积（CSA）、横截面惯性矩（CSMI）和强度应变指数（SSI）。vCtBMD、CSA和CSMI的准确度分别为1.1%、2.7%和6.4%，以变异系数（CV）表示。通过三点弯曲试验测量了其力学性能。对骨骼测量的方法误差为：断裂弯曲力7.3% ~ 10.1%，刚度15.0% ~ 15.2%,vCtBMD 2.0% ~ 2.4%, CSA 5.2% ~ 6.4%, CSMI 13.5% ~ 17.6%, SSI 8.9% ~ 18.1%， CSMI和CSA分别是股骨和胫骨断裂力的最佳解释变量，而CSI-I和CSMI分别是股骨和胫骨弹性模量的最佳预测变量，CSA与力学参数的相关性高于vCtBMD。在本研究的基础上，力学测试和pQCT测量与小鼠骨骼的生物力学研究相关，并证明了小鼠模型的使用，高分辨率pQCT在小鼠骨骼研究中比三点弯曲试验具有更好的精度。（骨23:155-161；1998）(C) 1998年Elsevier Science Int。版权所有。
We compared the mechanical and densitometric testing methods in evaluating the mechanical strength of mouse cortical hones, The femora and tibiae of 10 male mice (weight 32.8 +/- 4.0 g) mere utilized, Volumetric cortical bone mineral density (VCtBMD), cross-sectional cortical area at midshaft (CSA), cross-sectional moment of inertia (CSMI), and strength strain index (SSI) mere measured by peripheral quantitative computed tomography (pQCT), The precision of pQCT expressed as a coefficient of variation (CV) was 1.1%, 2.7%, and 6.4% for vCtBMD, CSA, and CSMI, respectively. The mechanical properties were measured by a three-point bending test. The method error measured from paired bones mas 7.3%-10.1% for breaking bending force, 15.0%-15.2% for stiffness, 2.0%-2.4% for vCtBMD, 5.2%-6.4% for CSA, 13.5%-17.6% for CSMI, and 8.9%-18.1% for SSI, CSMI and CSA mere found to be the best explanatory variables for the breaking force of femur and tibial respectively, while CSI-I and CSMI were the best predictors for the elastic modulus of femur and tibia, respectively, CSA had a higher correlation with mechanical parameters than VCtBMD. On the basis of this study, the mechanical tests and the pQCT measurements are relevant in biomechanical studies on mouse bones and justify the use of the murine model, High-resolution pQCT gives better precision than the three-point bending test in studies of mouse bones. (Bone 23:155-161; 1998) (C) 1998 by Elsevier Science Int. All rights reserved.