PbrWRKY42-PbrSOT13 module regulated sorbitol accumulation in the developing ‘Yali’ fruit after three-layer-paper bagging treatment
PbrWRKY42-PbrSOT13 module regulated sorbitol accumulation in the developing ‘Yali’ fruit after three-layer-paper bagging treatment
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DOI：
10.1016/j.scienta.2023.112705
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发表时间：
2024-02
影响因子：
4.3
通讯作者：
Luting Jia;Lanqing Li;Weiqi Luo;Xu Zhang;Lijuan Zhu;Ming Qian;Peng Gu;Yuhua Xie;Bing Yang
中科院分区：
文献类型：
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作者：
Luting Jia;Lanqing Li;Weiqi Luo;Xu Zhang;Lijuan Zhu;Ming Qian;Peng Gu;Yuhua Xie;Bing Yang
果实套袋作为一种常规的采前处理措施，能有效控制果实病虫害，改善果实外观品质，但抑制可溶性糖的积累。然而，直到最近，这种现象背后的分子机制还没有完全阐明。在本研究中，三层纸袋处理显著抑制了鸭梨果实中四种可溶性糖的积累。结合糖代谢相关基因的表达谱分析，筛选出一个位于质膜上的蛋白质PbrSOT 13，证实其参与山梨醇的内流和积累。分子动力学模拟结果表明，Asp 52和Arg 132残基在此过程中起关键作用。通过对PbrSOT 13启动子顺式作用元件和转录因子表达谱的联合分析，确定PbrWRKY 42为PbrSOT 13的上游调控因子。酵母单杂交和双荧光素酶检测结果表明，核PbrWRKY 42可以与PbrSOT 13启动子的W-box元件结合，激活其转录，从而促进山梨醇在果实中的积累。进一步的研究发现，PbrSOT 13和PbrWRKY 42基因的转录受到温度升高和光照时间缩短的抑制，这是套袋处理的结果。综上所述，我们的研究结果表明，三层纸套袋处理将改变周围的温度和光照的发展'鸭梨'果实，从而抑制山梨醇积累通过PbrWRKY 42-PbrSOT 13模块。
As a conventional preharvest handling practice, fruit bagging could control insects and diseases, and therefore improve the visual quality of pear, but suppress the accumulation of soluble sugar. However, the molecular mechanism underlying this phenomenon has not been fully elucidated until recently. In this study, the three-layer-paper bagging treatment considerably suppressed the accumulation of four soluble sugars in the developing ‘Yali’ fruit. In combination with the expression profiles of the differently expressed sugar-metabolism-related genes,PbrSOT13, encoding a plasma membrane-located protein, was selected and confirmed to participate in sorbitol influx and accumulation in pear. Notably, molecular dynamic simulation indicated that the Asp52and Arg132residues were pivotal in this process. After a conjoined analysis ofcis-acting elements inPbrSOT13promoter and the expression profiles of transcription factors, PbrWRKY42 was identified as a candidate upstream regulator ofPbrSOT13. The nuclear PbrWRKY42 could bind to the W-box element ofPbrSOT13promoter and then activate its transcription, as evidenced by yeast one-hybrid assay and dual-luciferase assay, thus enhancing sorbitol accumulation in fruit. Further study found that the transcription ofPbrSOT13andPbrWRKY42was suppressed by the elevated temperature and the reduced light-exposure period, which was the consequence of bagging treatment. Taken together, our results implied that three-layer-paper bagging treatment would alter temperature and light exposure around the developing ‘Yali’ fruit, and thus suppress sorbitol accumulation via the PbrWRKY42-PbrSOT13 module.