Expression of cytokeratin 17 mRNA in oral squamous cell carcinoma cells obtained by brush biopsy: preliminary results
Expression of cytokeratin 17 mRNA in oral squamous cell carcinoma cells obtained by brush biopsy: preliminary results
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DOI：
10.1111/j.1600-0714.2009.00748.x
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Wagner, W.
中科院分区：
文献类型：
--
作者：
Toyoshima, T.;Koch, F.;Wagner, W.
背景：本研究的目的是检测口腔鳞状细胞癌(OSCC)细胞中细胞角蛋白(CK)的表达，探讨CK在口腔鳞癌诊断中的意义。从细胞颗粒中提取mRNA，进行实时定量逆转录聚合酶链式反应(RT-qPCR)。结果：与正常口腔鳞癌组织相比，口腔鳞癌组织中有40例(76.9%)CK17、19例(36.5%)高表达CK17，19例(36.5%)未检测到CK20基因表达。与CK-19相比，52例患者CK-17基因表达水平均显著升高(P<；0.02)。此外，CK17在T1、T2期口腔鳞癌患者中显著升高(P<；0.03)，在无颈淋巴结转移患者中(P<；0.04)，在I期和II期患者中(P<；0.03和P<；0.05)，在分化较好的口腔鳞癌患者中(P<；0.05)。这项初步研究论证了CK17应用的可能性；然而，鼓励关键研究确认CK17作为刷检试验中口腔鳞癌的诊断标记物。
Background:The aim of this study was to determine the detection of cytokeratin (CK) mRNA in oral squamous cell carcinoma (OSCC) cells and to evaluate the CK relevance for OSCC diagnosis in a brush biopsy test.Methods:Fifty-two pairs of OSCC cells and normal oral mucosal cells were obtained by brush biopsy from OSCC patients. mRNA was extracted from cell pellets for real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). The over-expression levels of CK 17, CK 19 and CK 20 mRNA in OSCC cells were examined by SYBR green real-time RT-qPCR.Results:Compared to normal mucosal cells, the over-expression of CK 17 mRNA was detectable in 40 OSCC cells (76.9%), that of CK 19 mRNA in 19 (36.5%), while that of CK 20 mRNA was not detectable. Compared with CK 19, the mean value of CK 17 mRNA expression level was significantly higher in all 52 patients (P < 0.02). Moreover, the value of CK 17 was significantly higher in T1 and T2 OSCC patients (P < 0.03, respectively), in patients without metastases of neck lymph nodes (P < 0.04), in stage I and stage II patients (P < 0.03 and P < 0.05, respectively) and in well differentiated OSCC patients (P < 0.05).Conclusion:Brush biopsy properly serves for detection of CK mRNA using real-time RT-qPCR. This preliminary study demonstrates the CK 17 possibility for application; however, pivotal studies are encouraged to confirm CK 17 as a diagnostic marker of OSCC in a brush biopsy test.