Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.
Genome-Wide Association Study on Total Starch, Amylose and Amylopectin in Barley Grain Reveals Novel Putative Alleles.
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大麦籽粒中总淀粉、直链淀粉和支链淀粉的全基因组关联研究揭示了新的假定等位基因
DOI：
10.3390/ijms22020553
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发表时间：
2021-01-07
影响因子：
5.6
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Li M;Geng L;Xie S;Wu D;Ye L;Zhang G
谷物籽粒中淀粉的含量和组成与产量密切相关。在大麦中，与这些性状相关的基因或位点的鉴定研究很少。本研究旨在鉴定控制大麦籽粒总淀粉（TS）、直链淀粉（AC）和支链淀粉（AP）含量的基因或位点。一个大的基因型变异被发现在所有检查的淀粉性状。GWAS分析分别检测到13个、2个和10个影响TS、AC和AP的QTL，其中5个QTL与AP和TS含量共有。qTS-3.1、qAC-6.2和qAP-5.1可以分别解释TS、AC和AP的最大变化。四个假定的候选基因，即，HORVU 6 Hr 1G 087920、HORVU 5 Hr 1G 011230、HORVU 5 Hr 1G 011270和HORVU 5 Hr 1G 011280在淀粉快速积累的大麦籽粒中高表达。根据标记S5H_29297679的多态性，将100份大麦材料分为两类，其中93份材料具有GG等位基因，7份材料具有AA等位基因。此外，所鉴定的QTL的有利等位基因数与TS、AC、AP含量呈显著正相关。本研究所鉴定的位点或基因可用于大麦籽粒淀粉的遗传改良。
The content and composition of starch in cereal grains are closely related to yield. Few studies have been done on the identification of the genes or loci associated with these traits in barley. This study was conducted to identify the genes or loci controlling starch traits in barley grains, including total starch (TS), amylose (AC) and amylopectin (AP) contents. A large genotypic variation was found in all examined starch traits. GWAS analysis detected 13, 2, 10 QTLs for TS, AC and AP, respectively, and 5 of them were commonly shared by AP and TS content. qTS-3.1, qAC-6.2 and qAP-5.1 may explain the largest variation of TS, AC and AP, respectively. Four putative candidate genes, i.e., HORVU6Hr1G087920, HORVU5Hr1G011230, HORVU5Hr1G011270 and HORVU5Hr1G011280, showed the high expression in the developing barley grains when starch accumulates rapidly. The examined 100 barley accessions could be divided into two groups based on the polymorphism of the marker S5H_29297679, with 93 accessions having allele GG and seven accessions having AA. Moreover, significantly positive correlation was found between the number of favorable alleles of the identified QTLs and TS, AC, AP content. In conclusion, the identified loci or genes in this study could be useful for genetic improvement of grains starch in barley.