Inhibition of VCAM-1 expression in endothelial cells by reconstituted high density lipoproteins
Inhibition of VCAM-1 expression in endothelial cells by reconstituted high density lipoproteins
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DOI：
10.1006/bbrc.1997.7236
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发表时间：
1997-09-08
影响因子：
3.1
通讯作者：
Taramelli, D
中科院分区：
文献类型：
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作者：
Calabresi, L;Franceschini, G;Taramelli, D
血浆来源的高密度脂蛋白（HDL）被发现可以抑制细胞因子诱导的内皮细胞粘附分子的表达。在这里，我们使用由磷脂酰胆碱 (PC) 和三种不同的载脂蛋白制成的载脂蛋白特异性重组 HDL (rHDL) 来鉴定参与此效应的 HDL 成分。含有载脂蛋白 A-I (apoA-I)、二硫键连接形式的 apoA-I-Milano 变体或 apoA-II 的 rHDL 均能有效抑制 TNF α 或 LPS 刺激的 HUVEC 中血管细胞粘附分子 1 (VCAM-1) 和细胞间粘附分子 1 的表达。抑制作用在 0.1-1.0 mg/ml（蛋白质）范围内呈浓度依赖性。 PC脂质体轻微抑制TNFα诱导的VCAM-1表达（16% vs 各种rHDL 43-50%），而无脂载脂蛋白则没有影响。 HDL 的蛋白质成分通过一种相当非特异性的机制参与抑制 HUVEC 中的 VCAM-1 表达，因为具有明显不同一级结构的三种载脂蛋白表现出非常相似的活性。 (C) 1997 年学术出版社。
Plasma-derived high density lipoproteins (HDL) were found to inhibit cytokine-induced expression of endothelial cell adhesion molecules. Here we used apolipoprotein-specific reconstituted HDL (rHDL) made with phosphatidylcholine (PC) and three different apolipoproteins to identify the HDL components involved in this effect. rHDL containing apolipoprotein A-I (apoA-I), the disulfide-linked form of the apoA-I-Milano variant, or apoA-II, were all effective in inhibiting the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 in TNF alpha- or LPS-stimulated HUVEC. The inhibition was concentration dependent in the range of 0.1-1.0 mg/ml (protein). PC liposomes slightly depressed TNF alpha-induced VCAM-1 expression (16% vs 43-50% for the various rHDL), whereas the lipid-free apolipoproteins had no effect. The protein component of HDL is involved in the inhibition of VCAM-1 expression in HUVEC through a rather unspecific mechanism, as three apolipoproteins with remarkably different primary structure display very similar activity. (C) 1997 Academic Press.