USE OF MUTANTS TO DEMONSTRATE THE ROLE OF OXALIC-ACID IN PATHOGENICITY OF SCLEROTINIA-SCLEROTIORUM ON PHASEOLUS-VULGARIS
USE OF MUTANTS TO DEMONSTRATE THE ROLE OF OXALIC-ACID IN PATHOGENICITY OF SCLEROTINIA-SCLEROTIORUM ON PHASEOLUS-VULGARIS
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DOI：
10.1016/0885-5765(90)90010-u
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发表时间：
1990-09-01
影响因子：
2.7
通讯作者：
DAM, R
中科院分区：
文献类型：
--
作者：
GODOY, G;STEADMAN, JR;DAM, R
研究了草酸在菜豆白色霉菌--核盘菌（Sclerotinia sclerotiorum）致病性中的作用。通过紫外线诱变子囊孢子，获得了草酸产生缺陷的原养型突变株。缺乏草酸生产筛选马铃薯葡萄糖琼脂含有指示染料溴酚蓝的颜色变化。酶法、气相色谱法和高效液相色谱法表明，所选突变体在富含碳水化合物的培养基或豆花（一种天然底物）中不产生草酸。在生长室实验中，使用整个植物，茎和叶，并在实验室试验中使用豆荚，这些酸负突变体是非致病性的，而草酸生产的野生型和回复突变体是致病性的。草酸的生产诱导突变体生长在含有琥珀酸钠的营养培养基。在这种培养基上生长并接种在菜豆叶片上的突变体穿透并引发小病变。这些研究结果证实了草酸是一个致病性决定因子。
The role of oxalic acid in pathogenicity of the bean white mould fungus, Sclerotinia sclerotiorum, was investigated. Prototrophic mutants of the fungus deficient in oxalic acid production were obtained by UV irradiation of ascospores. Deficiency in oxalic acid production was screened by colour change on potato dextrose agar containing the indicator dye bromophenol blue. An enzymatic method, gas chromatography and high performance liquid chromatography indicated that the selected mutants did not produce oxalic acid in carbohydrate-rich media or bean blossoms, a natural substrate. In growth chamber experiments using whole plants, stems and leaves and in laboratory tests using pods, these acid minus mutants were nonpathogenic while the oxalic acid-producing wild type and revertant were pathogenic. Production of oxalic acid was induced when mutants were grown on nutrient media containing sodium succinate. Mutants grown on this medium and inoculated on bean leaves penetrated and incited small lesions. No association between pathogenicity and pectolytic enzymes was found. These studies present confirming evidence that oxalic acid is a pathogenicity determinant.