Emergence of the sensory nervous system as defined by Foxs1 expression
Emergence of the sensory nervous system as defined by Foxs1 expression
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DOI：
10.1111/j.1432-0436.2006.00154.x
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发表时间：
2007-06-01
期刊：
影响因子：
2.9
通讯作者：
Ernfors, Patrik
中科院分区：
文献类型：
--
作者：
Montelius, Andreas;Marmigere, Frederic;Ernfors, Patrik
外周感觉神经元来源于两个不同的结构，外胚层基板和神经嵴。在这里，我们建立叉头家族转录因子Foxs1作为早期感觉神经元标志物。早期胚胎Foxs1表达存在于所有感觉神经系统中，而不考虑细胞来源，但在其他基板和神经嵴来源的细胞类型中未发现。Foxs1的表达在躯干的感觉神经元前体中开启。在未分化的多能神经嵴细胞（NCCs）中，Sox10的表达与Foxs1相排斥。Foxs1表达的获得用于研究背根神经节（DRG）感觉神经元的出现。在18体期，在前体细胞背唇处发现了表达NCCs的迁移先驱Foxs1。这些细胞表现出有限的增殖，并在聚结神经节的腹侧形成一个簇，周围是Foxs1(-)/Sox10（+）迁移的NCCs，保持了较高的增殖率。Foxs1(-)/Sox10（+）前体的感觉神经发生发生在浓缩DRG内，始于神经原素-1 （Ngn1）和Brn3a的表达。这些数据定义了具有不同分子特征的感觉神经系统神经元前体的顺序出现，从迁移期间开始，并在DRG凝聚后持续很长时间。
Peripheral sensory neurons are derived from two distinct structures, the ectodermal placodes and the neural crest. Here, we establish the forkhead family transcription factor Foxs1 as an early sensory neuronal marker. Early embryonic Foxs1 expression was present in all the sensory nervous system regardless of cellular origin, but was not found in other placode and neural crest-derived cell types. Foxs1 expression was turned on in the sensory neuron precursors of the trunk. Consistently, expression of Sox10, that is present in undifferentiated multipotent neural crest cells (NCCs), was mutually exclusive to Foxs1. Acquirement of Foxs1 expression was used to study the emergence of the dorsal root ganglion (DRG) sensory neurons. Migrating pioneering Foxs1 expressing NCCs were found at the anterior dorsal somitic lip at the 18-somite stage. These cells showed limited proliferation and migrated to form a cluster in the ventral aspect of the coalescing ganglion, surrounded by Foxs1(-)/Sox10(+) migrating NCCs retaining a high rate of proliferation. Sensory neurogenesis of the Foxs1(-)/Sox10(+) precursors occurred within the condensed DRG starting with neurogenin-1 (Ngn1) and Brn3a expression. These data define a sequential emergence of neuronal precursors of the sensory nervous system with different molecular characteristics, starting during migration and continuing well after DRG condensation.