Conservation of nif sequences in Frankia
Conservation of nif sequences in Frankia
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DOI：
10.1007/bf00339587
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发表时间：
1988-08
期刊：
影响因子：
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通讯作者：
P. Normand;P. Simonet;R. Bardin
中科院分区：
文献类型：
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作者：
P. Normand;P. Simonet;R. Bardin
用苜蓿根瘤菌、肺炎克雷伯菌和Frankia菌株Ar13的nifHDK探针与Frankia总DNA进行Southern杂交。注意到菌株之间的差异在于杂交限制性片段的大小。这些差异更明显的胡颓子亲和菌株比Alnus或木麻黄亲和菌株。利用构建的EUN 1f、HRN 18a、CeD和ACoN 24d基因库，分离出与之杂交的限制性内切酶片段，用于后续的作图和比较。其中，Hzone基因的保守性最高，而HandnifD基因的保守性最高。Frankia Ar13株的henifHopen阅读框（ORF）全长861 bp，编码287个氨基酸。这些核酸和氨基酸序列与其它已克隆的nifH序列的比较表明，Franki与Anabaena和Azotocarpus spp最相似。和K.肺炎球菌，与革兰氏阳性巴氏梭菌和古细菌伏特甲烷球菌最不相似。
Southern blots ofFrankiatotal DNAs were hybridized withnifHDKprobes fromRhizobium meliloti, Klebsiella pneumoniaeandFrankiastrain Arl3. Differences between strains were noted in the size of the hybridizing restriction fragments. These differences were more pronounced amongElaeagnus-compatible strains than amongAlnus- orCasuarina-compatible strains. Gene banks constructed forFrankiastrains EUN1f, HRN18a, CeD and ACoN24d were used to isolatenif-hybridizing restriction fragments for subsequent mapping and comparisons. ThenifHzone had the highest sequence conservation and thenifHandnifDgenes were found to be contiguous. The complete nucleotide sequence of thenifHopen reading frame (ORF) from Frankia strain Arl3 is 861 bp in length and encodes a polypeptide of 287 amino acids. Comparisons of these nucleic acid and amino acid sequences with other publishednifHsequences suggest thatFrankiais most similar toAnabaenaandAzotobacterspp. andK. pneunoniaeand least similar to the Gram-positiveClostridium pasteurianumand to the archaebacteriumMethanococcus voltae.