Mitochondrial D-loop 3′ (CA)n repeat polymorphism:: Optimization of analysis and population data
Mitochondrial D-loop 3′ (CA)n repeat polymorphism:: Optimization of analysis and population data
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DOI：
10.1002/elps.1150181523
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发表时间：
1997-12-01
期刊：
影响因子：
2.9
通讯作者：
Krause, D
中科院分区：
文献类型：
--
作者：
Szibor, R;Michael, M;Krause, D
我们报告了一个在线粒体控制区3'区的二核苷酸重复多态性。使用一个新的引物组获得的片段可以可靠地分离聚丙烯酰胺凝胶电泳（PAGE）使用非变性凝胶。在银染凝胶上共检测到5个等位基因[（CA），~（CA），I]。90 bp产物对应于等位基因5。从一个非洲和三个欧洲人口的样本进行了表征。不同人群的单等位基因发生率和等位基因分布存在显著差异。这些差异是在三个欧洲班图人和一个非洲班图人之间发现的。对于特定的法医问题，线粒体CA重复是非常合适的。德国、匈牙利、俄罗斯联邦和喀麦隆群体的基因多样性分别为0.36、0.40、0.34、0.52。
We report a dinucleotide repeat polymorphism in the 3' area of the mitochondrial control region. The fragments obtained using a new primer set could be reliably separated by polyacrylamide gel electrophoresis (PAGE) using nondenaturing gels. A total of five alleles [(CA), to (CA),I were detected on silver-stained gels. The 90 bp product corresponds to allele 5. Samples from one African and three European populations were characterized. Significant differences could be demonstrated as to the incidence of single alleles and allele distributions in different populations. These differences were found between the three European and one African Bantu population. For specific forensic questions the mitochondrial CA repeat is well suited. Gene diversities in populations of Germany, Hungary, the Russian Federation and Cameroon were 0.36, 0.40, 0.34, 0.52, respectively.