"Mycoplasmal Antigen Modulation," a Novel Surface Variation Suggested for a Lipoprotein Specifically Localized on Mycoplasma mobile
"Mycoplasmal Antigen Modulation," a Novel Surface Variation Suggested for a Lipoprotein Specifically Localized on Mycoplasma mobile
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“支原体抗原调节”，一种针对支原体移动体上特异性定位的脂蛋白提出的新型表面变异
DOI：
10.1007/s00284-012-0090-y
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发表时间：
2012
影响因子：
2.6
通讯作者：
Miyata M
中科院分区：
文献类型：
--
作者：
Wu HN;Kawaguchi C;Nakane D;Miyata M
移动的支原体是淡水鱼的一种病原体，很容易滑过表面，在鱼鳃上定植，并引起坏死。细胞表面分为三部分：头部、颈部和身体。移动的可变表面蛋白（Mvsps）定位于这些部分中的每一个可能参与表面变化，包括相位变化和抗原性变化，尽管没有证据存在。在本研究中，我们通过关注最大的Mvsp MvspI来研究这种可能性。免疫荧光显微镜显示MvspI在所有细胞的表面上表达。当以超过0.8 nM的浓度加入抗MvspI抗体时，观察到MvspI随时间降低。与抗体培养72小时后，MvspI的荧光强度和量分别下降至13%和39%，与无抗体生长的细胞相比。这些变化通过去除抗体而逆转。当使用靶向其他Mvsps的另一种抗体时，未观察到这种效应，表明这种降低对MvspI和抗体之间的关系具有特异性。细胞生长也受到抗体的抑制，但MvspI的减少不能解释为MvspI阴性变体的选择性生长或其他条件下的生长抑制。由抗体结合引起的MvspI的减少可能表明一种新型的表面变异，在此称为“支原体抗原调节”。
Mycoplasma mobile,a pathogen of freshwater fish, glides easily across surfaces, colonizes on the fish gill, and causes necrosis. The cell surface is differentiated into three parts: the head, neck, and body. Mobile variable surface proteins (Mvsps) localizing at each of these parts may be involved in surface variation including phase variation and antigenic variation, although no proof exists. In this study, we examined this possibility by focusing on MvspI, the largest Mvsp. Immunofluorescence microscopy showed that MvspI is expressed on the surfaces of all cells. When anti-MvspI antibody was added at concentrations over 0.8 nM, MvspI was observed to decrease over time. After 72 h of cultivation with the antibody, the fluorescence intensity and amount of MvspI decreased up to 13 and 39%, respectively, compared to those of cells grown without antibody. These changes were reversed by the removal of the antibody. Such effects were not observed when another antibody targeting other Mvsps was used, suggesting that the decrease is specific to the relationship between MvspI and the antibody. Cell growth was also inhibited by the antibody, but the decrease in MvspI could not be explained by the selective growth of MvspI-negative variants or by the inhibition of growth with other conditions. The decrease in MvspI caused by the antibody binding may suggest a novel type of surface variation, designated here as “mycoplasmal antigen modulation.”