Habitual Light-intensity Physical Activity and ASC Methylation in a Middle-aged Population
Habitual Light-intensity Physical Activity and ASC Methylation in a Middle-aged Population
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中年人群习惯性轻强度体力活动与 ASC 甲基化
DOI：
10.1055/a-0965-1374
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发表时间：
2019
影响因子：
2.5
通讯作者：
Tanaka K
中科院分区：
文献类型：
--
作者：
Nishida Y;Hara M;Higaki Y;Taguchi N;Nakamura K;Nanri H;Horita M;Shimanoe C;Yasukata J;Miyoshi N;Yamada Y;Higashimoto K;Soejima H;Tanaka K
凋亡相关的斑点样蛋白含有一个caspase募集结构域（ASC），在外周血单核细胞（PBMC）的炎性细胞因子合成中起重要作用，其CpG位点的甲基化水平升高可抑制ASC的表达。本研究调查了用轻强度体力活动（LPA）或中等强度体力活动（MVPA）取代久坐时间对中年人ASC甲基化的纵向关联。我们调查了1238名参与基于人群的队列研究的基线和5年随访调查的个体。使用加速度计客观测量久坐、LPA和MVPA时间。通过焦磷酸测序测量PBMC中的ASC甲基化。使用多元线性回归和采用等时替代模型，调整潜在混杂因素后，分析久坐时间、LPA和MVPA变化对ASC甲基化变化的纵向关联。用每天60分钟的LPA代替久坐时间与1.17倍（95%置信区间1.07，1.27）的ASC甲基化水平相关（7个CpG位点的平均值，P<0.001）。然而，MVPA没有观察到这种影响。这些结果表明，用LPA替代久坐时间可能与增加的（有利的）ASC甲基化相关，ASC甲基化是全身性炎症的潜在生物标志物。
Apoptosis-associated, speck-like protein containing a caspase recruitment domain (ASC) plays an important role in inflammatory cytokine synthesis in peripheral blood mononuclear cells (PBMCs), and the expression of ASC is suppressed by increased methylation of its CpG sites. The current study investigated the longitudinal association of replacing sedentary time with light-intensity physical activity (LPA) or moderate to vigorous-intensity physical activity (MVPA) on the ASC methylation in middle-aged people. We investigated 1 238 individuals who participated in baseline and 5-year follow-up surveys of a population-based cohort study. Sedentary, LPA and MVPA time were objectively measured using accelerometers. ASC methylation in PBMCs was measured by pyrosequencing. Using a multiple linear regression and employing an isotemporal substitution model, the longitudinal associations of changes in the sedentary time, LPA and MVPA on the changes in the ASC methylation were analyzed after adjusting for potential confounders. Substituting 60 min per day of LPA for sedentary time was associated with 1.17 times (95% confidence interval 1.07, 1.27) higher ASC methylation levels (mean of 7 CpG sites,P<0.001). However, such effects were not seen for MVPA. These results suggest that substituting LPA for sedentary time may be linked with increased (favorable) ASC methylation as a potential biomarker of systemic inflammation.
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影响因子：
6
作者：
Butts B;Gary RA;Dunbar SB;Butler J
通讯作者：
Butler J
DOI：
10.1097/00008483-199911000-00018
发表时间：
1999-05
期刊：
JAMA
影响因子：
--
作者：
J. Smith;R. Dykes;J. E. Douglas;G. Krishnaswamy;S. Berk
通讯作者：
J. Smith;R. Dykes;J. E. Douglas;G. Krishnaswamy;S. Berk
影响因子：
4.7
作者：
Imaeda N;Goto C;Tokudome Y;Hirose K;Tajima K;Tokudome S
通讯作者：
Tokudome S
影响因子：
3.6
作者：
Kumahara, H;Schutz, Y;Tanaka, H
通讯作者：
Tanaka, H
长周期加速度计监测显示体力活动在超重和肥胖中的作用
影响因子：
4.9
作者：
M. Yoshioka;M. Yoshioka;M. Ayabe;Takuya Yahiro;Hiroyuki Higuchi;Yasuki Higaki;J. St;Hideo Miyazaki;Yutaka Yoshitake;M. Shindo;Hiroaki Tanaka
通讯作者：
Hiroaki Tanaka