Enzymatic reactions involving sulfate, sulfite, selenate, and molybdate.
Enzymatic reactions involving sulfate, sulfite, selenate, and molybdate.
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涉及硫酸盐、亚硫酸盐、硒酸盐和钼酸盐的酶促反应。
DOI：
10.1016/s0021-9258(18)64689-7
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
R. Bandurski
中科院分区：
文献类型：
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作者：
L. G. Wilson;R. Bandurski
材料和方法硫酸化酶测定-ATP-硫酸化酶的活性是通过在磷酸盐和无机焦磷酸酶存在下从ATP中酶促释放无机磷酸盐来测定的。反应混合物以pmol计含有：ATP，5; NazMo 〇 4，5; MgCl 2，1; Tris-HCl缓冲液，pH 7.5，50; EDTA，0.3;纯化的焦磷酸酶，1.8yg，和硫酸化酶，总体积为0.50 ml。一个单位的酶被定义为在30 ° C下在60分钟内产生1皮摩尔无机磷酸盐的量。反应随时间线性进行，直到90%的ATP被水解，并且是酶浓度的线性函数（图1）。通过煮沸、酒精或稀释来停止酶活性。三-
MATERIALS AND METHODSXulfurylase zlssay-The activity of ATP-sulfurylase was determined by following the enzymatic liberation of inorganic phosphate from ATP in the presence of molybdate and inorganic pyrophosphatase. The reaction mixture contained in pmoles: ATP, 5; NazMo04, 5; MgC12, 1; Tris-HCl buffer, pH 7.5, 50; EDTA, 0.3; purified pyrophosphatase, 1.8 pg., and the sulfurylase in a total volume of 0.50 ml. A unit of enzyme was defined as that amount which produced 1 pmole of inorganic phosphate in 60 minutes at 30”. The reaction proceeded linearly with time until 90 per cent of the ATP was hydrolyzed and was a linear function of enzyme concentration (Fig. 1). Enzymatic activity was halted by boiling, by alcohol, or by dilution. Tri-