Interspecific DNA transformation in Drosophila.
Interspecific DNA transformation in Drosophila.
复制标题
果蝇种间 DNA 转化。
DOI：
10.1073/pnas.81.23.7515
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发表时间：
1984
影响因子：
11.1
通讯作者：
Hartl,DL
中科院分区：
文献类型：
--
作者：
Scavarda,NJ;Hartl,DL
通过显微注射将含有黑腹果蝇野生型rosy(ry+)基因的DNA片段导入繁殖分离种拟果蝇的种子系，并整合到拟果蝇基因组中。转化是由转座因子P介导的，转座因子P存在于大多数自然种群的黑腹盘藻基因组中，而不存在于拟盘藻的基因组中。Rubin和Spradling[Rubin，G.M.&Spradling，A.C.(1982)Science 218,348-353]此前已经证明，位于P元件识别序列两侧的ry+DNA片段可以转化黑腹果蝇的种系。在拟南芥中的成功转化表明，P元件继续作为转座元件在拟南芥基因组中发挥作用。此外，尽管自这些物种的进化分化以来，估计有100万到500万年的生殖隔离，但黑腹隐翅虫的ry+基因在仿生丝虫的基因组中发挥了作用，产生了正常的眼睛颜色。种间DNA转化为研究遗传差异对相关但繁殖隔离物种基因表达的影响提供了一种有用的方法。
A DNA fragment that includes the wild-type rosy (ry+) gene of Drosophila melanogaster has been introduced by microinjection into the germ line of the reproductively isolated species Drosophila simulans and incorporated into the D. simulans genome. Transformation was mediated by the transposable element P, which occurs in the genome of most natural populations of D. melanogaster but not in D. simulans. Rubin and Spradling [Rubin, G.M. & Spradling, A.C. (1982) Science 218, 348-353] have previously shown that the ry+ DNA fragment, which is flanked by recognition sequences of P element, can transform the germ line of D. melanogaster. Successful transformation in D. simulans indicates that the P element continues to function as a transposable element in the D. simulans genome. Moreover, the ry+ gene of D. melanogaster functions in the genome of D. simulans to produce normal eye color, despite the estimated 1 to 5 million yr of reproductive isolation since the evolutionary divergence of these species. Interspecific DNA transformation provides a useful method for the study of genetic differences affecting gene expression among related but reproductively isolated species.