Integrated Cross-Sectional Multiplex Serosurveillance of IgG Antibody Responses to Parasitic Diseases and Vaccines in Coastal Kenya
Integrated Cross-Sectional Multiplex Serosurveillance of IgG Antibody Responses to Parasitic Diseases and Vaccines in Coastal Kenya
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DOI：
10.4269/ajtmh.19-0365
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Priest, Jeffrey W.
中科院分区：
文献类型：
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作者：
Njenga, Sammy M.;Kanyi, Henry M.;Priest, Jeffrey W.
准确且具有成本效益地确定同时流行感染发生的地区将使公共卫生管理者能够确定实施综合控制计划的机会。在肯尼亚沿海横断面淋巴丝虫病调查中收集的干血点用于探索性综合检测针对几种寄生虫感染（班克罗夫蒂虫、曼氏血吸虫、疟原虫、蛔虫和粪类圆线虫）抗原的 IgG 抗体，以及检测针对疫苗可预防疾病 (VPD) 的免疫制剂的反应（麻疹、白喉和破伤风）使用多重微珠测定 (MBA) 平台。在前哨位点的病原体和抗原的抗体反应中观察到高度异质性。恩道岛的丝虫抗原抗体血清阳性率普遍较高（P < 0.0001），与其他社区相比，该岛的丝虫抗原血症患病率也最高。 Kilifi 县和 Kwale 县对疟原虫物种抗原环子孢子蛋白 (CSP) 和裂殖子表面蛋白 1 (MSP-1)(19) 的抗体反应较高，Jaribuni 社区显示出较高的总体平均血清阳性率 (P < 0.0001)。泰塔-塔维塔县的 Kimorigo 社区是唯一检测到针对曼氏沙门氏菌 Sm25 重组抗原的抗体反应的地区。类圆线虫抗原 NIE 的血清阳性率在 3% 至 26% 之间，并且研究群体中针对蛔虫抗原的免疫反应存在高度异质性。不同社区之间 VPD 血清阳性率存在差异。夸勒县的破伤风血清防护普遍低于其他县。这项研究表明，MBA 有望快速综合监测流行地区对公共卫生具有重要意义的感染趋势。
Accurate and cost-effective identification of areas where co-endemic infections occur would enable public health managers to identify opportunities for implementation of integrated control programs. Dried blood spots collected during cross-sectional lymphatic filariasis surveys in coastal Kenya were used for exploratory integrated detection of IgG antibodies against antigens from several parasitic infections (Wuchereria bancrofti, Schistosoma mansoni, Plasmodium spp., Ascaris lumbricoides, and Strongyloides stercoralis) as well as for detection of responses to immunizing agents used against vaccine-preventable diseases (VPDs) (measles, diphtheria, and tetanus) using a multiplex bead assay (MBA) platform. High heterogeneity was observed in antibody responses by pathogen and antigen across the sentinel sites. Antibody seroprevalence against filarial antigens were generally higher in Ndau Island (P < 0.0001), which also had the highest prevalence of filarial antigenemia compared with other communities. Antibody responses to the Plasmodium species antigens circumsporozoite protein (CSP) and merozoite surface protein-1 (MSP-1)(19) were higher in Kilifi and Kwale counties, with Jaribuni community showing higher overall mean seroprevalence (P < 0.0001). Kimorigo community in Taita-Taveta County was the only area where antibody responses against S. mansoni Sm25 recombinant antigen were detected. Seroprevalence rates to Strongyloides antigen NIE ranged between 3% and 26%, and there was high heterogeneity in immune responses against an Ascaris antigen among the study communities. Differences were observed between communities in terms of seroprevalence to VPDs. Seroprotection to tetanus was generally lower in Kwale County than in other counties. This study has demonstrated that MBA holds promise for rapid integrated monitoring of trends of infections of public health importance in endemic areas.