Detection of telomerase activity in malignant and nonmalignant skin conditions
Detection of telomerase activity in malignant and nonmalignant skin conditions
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DOI：
10.1111/1523-1747.ep12345811
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发表时间：
1996-04-01
影响因子：
6.5
通讯作者：
Shay, JW
中科院分区：
文献类型：
--
作者：
Taylor, RS;Ramirez, RD;Shay, JW
端粒是线性染色体的末端区域，在正常的体细胞中，端粒的长度随着细胞的连续分裂而缩短，端粒酶是一种核糖核蛋白酶，在永生细胞和生殖细胞中维持端粒的长度。虽然存在于人类胎儿组织中，但出生后不久，除了生殖细胞、造血细胞和大多数人类原发性肿瘤外，端粒酶活性是不可检测的。在本研究中，我们显示端粒酶活性存在于77例基底细胞癌中的73例，18例非转移性皮肤鳞状细胞癌中的15例，以及7例皮肤黑色素瘤中的6例中，与在防晒皮肤、阳光损伤皮肤、银屑病皮损皮肤和毒藤皮炎皮损中检测到的极低水平相比，与防晒皮肤相比，具有增加的端粒酶活性水平，尽管低于在肿瘤组织中检测到的水平。因为端粒酶活性可以在皮肤的炎性病变中发现，这表明端粒酶活性并不总是与恶性表型相关。另外，我们发现新生儿包皮的表皮具有端粒酶活性，这表明端粒酶由表皮细胞组成性表达。在暴露于阳光下的皮肤中发现端粒酶活性水平高于未暴露的皮肤，这表明环境因素可能会调节端粒酶活性。
Telomeres are the end regions of linear chromosomes, and in normal somatic cells the lengths of telomeres shorten with successive cell divisions, Telomerase, a ribonucleoprotein enzyme, maintains the length of telomeres in immortal and germline cells. Although present in human fetal tissues, shortly after birth telomerase activity is not detectable except in germline cells, hematopoietic cells, and most human primary tumors. In the present study we show telomerase activity to be present in 73 of 77 basal cell carcinomas, 15 of 18 nonmetastatic cutaneous squamous cell carcinomas, and 6 of 7 cutaneous melanomas, contrasting with extremely low levels detected in sun-protected skin, Sun-damaged skin, psoriatic lesional skin, and skin from lesions of poison ivy dermatitis, however, have increased levels of telomerase activity compared to sun-protected skin, although less than that detected in tumor tissue, Because telomerase activity can be found in inflammatory lesions of the skin, this indicates that telomerase activity does not always correlate with the malignant phenotype, In addition, we show that telomerase activity is localized to the epidermis of newborn foreskin, which suggests that telomerase is expressed constitutively by cells in the epidermis. Finding higher levels of telomerase activity in sun-exposed skin compared to nonexposed skin suggests that environmental factors may modulate telomerase activity.