Anillin regulates epithelial cell mechanics by structuring the medial-apical actomyosin network
Anillin regulates epithelial cell mechanics by structuring the medial-apical actomyosin network
复制标题
DOI：
10.7554/elife.39065
复制
发表时间：
2019-01-31
期刊：
影响因子：
7.7
通讯作者：
Miller, Ann L.
中科院分区：
文献类型：
--
作者：
Arnold, Torey R.;Shawky, Joseph H.;Miller, Ann L.
细胞力量在发育过程中塑造生物体，而细胞力学的失调可能会促进疾病。在这里，我们调查如何肌动球蛋白支架蛋白苯胺有助于非洲爪蟾胚胎上皮力学。当苯胺醛过表达时，黏着斑蛋白向细胞-细胞连接的机械敏感性募集增加，表明苯胺醛促进连接张力。然而，接头激光消融意外地显示，接头回缩更快时，苯胺被耗尽和更慢时，苯胺被过表达。统一这些研究结果，我们表明，苯胺调节内侧顶端肌动球蛋白。内侧-顶端激光消融支持了这样的结论：张力储存在上皮细胞的顶端表面，而苯胺林促进了储存在该网络中的张力。最后，我们表明，苯胺对细胞力学的影响影响组织范围的力学。这些结果揭示了作为上皮力学的关键调节剂的苯胺青霉素，并为今后研究苯胺青霉素如何可能有助于发育和疾病中的机械事件奠定了基础。
Cellular forces sculpt organisms during development, while misregulation of cellular mechanics can promote disease. Here, we investigate how the actomyosin scaffold protein anillin contributes to epithelial mechanics in Xenopus laevis embryos. Increased mechanosensitive recruitment of vinculin to cell- cell junctions when anillin is overexpressed suggested that anillin promotes junctional tension. However, junctional laser ablation unexpectedly showed that junctions recoil faster when anillin is depleted and slower when anillin is overexpressed. Unifying these findings, we demonstrate that anillin regulates medial-apical actomyosin. Medial-apical laser ablation supports the conclusion that that tensile forces are stored across the apical surface of epithelial cells, and anillin promotes the tensile forces stored in this network. Finally, we show that anillin's effects on cellular mechanics impact tissue-wide mechanics. These results reveal anillin as a key regulator of epithelial mechanics and lay the groundwork for future studies on how anillin may contribute to mechanical events in development and disease.