Cyclic-diGMP signal transduction systems in Vibrio cholerae:: modulation of rugosity and biofilm formation
Cyclic-diGMP signal transduction systems in Vibrio cholerae:: modulation of rugosity and biofilm formation
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DOI：
10.1111/j.1365-2958.2006.05106.x
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Yildiz, FH
中科院分区：
文献类型：
--
作者：
Lim, B;Beyhan, S;Yildiz, FH
环状二鸟苷酸 (c-diGMP) 是调节多种微生物细胞表面特性的第二信使。细胞中 c-diGMP 的浓度由二鸟苷酸环化酶和磷酸二酯酶的相反活性控制，这两个酶分别由含有 GGDEF 和 EAL 结构域的蛋白质执行。在这项研究中，我们报告说，与平滑变体相比，霍乱弧菌皱纹变体中 c-diGMP 的细胞水平更高。通过过表达具有 GGDEF 或 EAL 结构域的蛋白质来调节细胞 c-diGMP 水平，分别增加或减少集落粗糙度。编码具有 GGDEF 或 EAL 结构域的蛋白质的几个基因在两种霍乱弧菌变种之间存在差异表达。这些基因（cdgA-E、rocS 和 mbaA）无效突变体的产生和表征表明，皱纹集落形成、胞外多糖产生、运动性和生物膜形成受其作用控制。此外，上位性分析表明，cdgC、rocS 和 mbaA 在收敛途径中发挥作用，调节皱纹和平滑变体的表型特性，并且是 VpsR、VpsT 和 HapR 信号转导途径的一部分。
Cyclic di-guanylic acid (c-diGMP) is a second messenger that modulates the cell surface properties of several microorganisms. Concentrations of c-diGMP in the cell are controlled by the opposing activities of diguanylate cyclases and phosphodiesterases, which are carried out by proteins harbouring GGDEF and EAL domains respectively. In this study, we report that the cellular levels of c-diGMP are higher in the Vibrio cholerae rugose variant compared with the smooth variant. Modulation of cellular c-diGMP levels by overexpressing proteins with GGDEF or EAL domains increased or decreased colony rugosity respectively. Several genes encoding proteins with either GGDEF or EAL domains are differentially expressed between the two V. cholerae variants. The generation and characterization of null mutants of these genes (cdgA-E, rocS and mbaA) revealed that rugose colony formation, exopolysaccharide production, motility and biofilm formation are controlled by their action. Furthermore, epistasis analysis suggested that cdgC, rocS and mbaA act in convergent pathways to regulate the phenotypic properties of the rugose and smooth variants, and are part of the VpsR, VpsT and HapR signal transduction pathway.