The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesoderm
The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesoderm
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DOI：
10.1016/s1534-5807(01)00040-5
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发表时间：
2001-09-01
影响因子：
11.8
通讯作者：
Buckingham, ME
中科院分区：
文献类型：
--
作者：
Kelly, RG;Brown, NA;Buckingham, ME
心脏动脉极的发育是心脏发生的关键步骤，但其胚胎学起源仍不清楚。我们分析了转基因小鼠品系，其中在胚胎右心室和心脏流出道以及邻近的内脏和咽中胚层中观察到β-半乳糖苷酶活性。 nlacZ 转基因已整合到成纤维细胞生长因子 10 (Fgf10) 基因的上游，与咽中胚层中 Fgf10 的表达模式进行比较表明转基因受 Fgf10 调控序列控制。 Dil 标记显示，在胚胎第 8.25 天和第 10.5 天之间，细胞从咽弓区域逐渐移动到正在生长的心管中。这些数据表明动脉极心肌起源于经典心区之外。
Development of the arterial pole of the heart is a critical step in cardiogenesis, yet its embryological origin remains obscure. We have analyzed a transgenic mouse line in which beta-galactosidase activity is observed in the embryonic right ventricle and outflow tract of the heart and in contiguous splanchnic and pharyngeal mesoderm. The nlacZ transgene has integrated upstream of the fibroblast growth factor 10 (Fgf10) gene and comparison with the expression pattern of Fgf10 in pharyngeal mesoderm indicates transgene control by Fgf10 regulatory sequences. Dil labeling shows a progressive movement of cells from the pharyngeal arch region into the growing heart tube between embryonic days 8.25 and 10.5. These data suggest that arterial pole myocardium originates outside the classical heart field.