Tumorigenic Potential of Mononucleated Small Cells of Hodgkin Lymphoma Cell Lines
Tumorigenic Potential of Mononucleated Small Cells of Hodgkin Lymphoma Cell Lines
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DOI：
10.2353/ajpath.2010.100089
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发表时间：
2010-12-01
影响因子：
6
通讯作者：
Morii, Eiichi
中科院分区：
文献类型：
--
作者：
Ikeda, Jun Ichiro;Mamat, Suhana;Morii, Eiichi
肿瘤细胞与肿瘤发生的潜在仅限于一个小细胞群被称为癌症干细胞(二者)二者产生二者和非二者而非二者不产生二者二者没有被确定m任何恶性淋巴瘤霍奇金淋巴瘤(HL)是一个主要是B细胞肿瘤诊断的多核的存在(里德斯特恩伯格RS)可见混有何杰金氏病细胞的细胞有明显核仁和各种炎症细胞细胞的致瘤潜能单一核(S)与多个细胞核和细胞(M)这可能是相当于霍奇金和RS细胞分别在霍奇金淋巴瘤细胞株L1236检查和L428文化的单一细胞产生两个年代和M细胞而M细胞培养时只产生M细胞培养在甲基纤维素或接种到点头/ SCID鼠种群数量和肿瘤大小都是大S比M细胞浓度的细胞内活性氧(ROS)在低水平的一部分在HL临床样本中，FoxO3a在单核霍奇金细胞中表达，而在多核RS细胞中不表达。这些发现表明，较小的细胞或霍奇金细胞显示低ROS浓度和高FoxO3a表达水平可能是HL CSCs的候选细胞（Am J Pathol 2010 177 3081-3084 DOI 10 2353/ajpath 2010 100089）。
Tumor cells with tumorigenic potential are limited to a small cell population known as cancer stem cells (CSCs) CSCs yield both CSCs and non CSCs whereas non CSCs do not yield CSCs CSCs have not been identified m any malignant lymphomas Hodgkin lymphoma (HL) is a mostly B cell neoplasm that can be diagnosed by the presence of multinucleated (Reed Sternberg RS) cells admixed with Hodgkin cells with distinct nucleoli and various inflammatory cells Here the tumorigenic potential of cells with a single nucleus (S) and cells with multiple nuclei (M) which may be equivalent to Hodgkin and RS cells respectively was examined in HL cell lines L1236 and L428 Cultures of single S cells yielded both S and M cells whereas M cell cultures yielded only M cells When either cultured in methylcellulose or inoculated into NOD/SCID mice the colony number and tumor size were both larger in S than in M cells Concentrations of intracellular reactive oxygen species (ROS) were at low levels in a portion of S cells that abundantly expressed FoxO3a a transcription factor that regulates ROS degrading enzymes In clinical samples of HL FoxO3a was expressed in mononuclear Hodgkin cells but not in multinucleated RS cells These findings suggest that smaller cells or Hodgkin cells that show low ROS concentrations and high FoxO3a expression levels might be candidates for HL CSCs (Am J Pathol 2010 177 3081-3084 DOI 10 2353/ajpath 2010 100089)