Imaging and characterization of stretch-induced ATP release from alveolar A549 cells
Imaging and characterization of stretch-induced ATP release from alveolar A549 cells
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DOI：
10.1113/jphysiol.2012.244145
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发表时间：
2013-03-01
影响因子：
5.5
通讯作者：
Sokabe, Masahiro
中科院分区：
文献类型：
--
作者：
Grygorczyk, Ryszard;Furuya, Kishio;Sokabe, Masahiro
细胞和组织水平的机械转导通常涉及ATP的释放和嘌呤能信号级联的激活。在肺中，拉伸是一种重要的物理刺激，但其对ATP释放的影响、潜在的释放机制和转导途径尚不清楚。本研究采用实时荧光素酶生物发光成像结合同步红外成像技术，研究了单向拉伸对人肺泡A549细胞ATP释放的影响，以监测细胞拉伸程度并鉴定ATP释放细胞。在subconfluent中(
Mechano-transduction at cellular and tissue levels often involves ATP release and activation of the purinergic signalling cascade. In the lungs, stretch is an important physical stimulus but its impact on ATP release, the underlying release mechanisms and transduction pathways are poorly understood. Here, we investigated the effect of unidirectional stretch on ATP release from human alveolar A549 cells by real-time luciferinluciferase bioluminescence imaging coupled with simultaneous infrared imaging, to monitor the extent of cell stretch and to identify ATP releasing cells. In subconfluent (