INTERLEUKIN-1 RECEPTOR ANTAGONIST PREVENTS SEPSIS-INDUCED INHIBITION OF PROTEIN-SYNTHESIS
INTERLEUKIN-1 RECEPTOR ANTAGONIST PREVENTS SEPSIS-INDUCED INHIBITION OF PROTEIN-SYNTHESIS
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DOI：
10.1152/ajpendo.1994.267.5.e636
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发表时间：
1994-11-01
影响因子：
5.1
通讯作者：
VARY, T
中科院分区：
文献类型：
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作者：
COONEY, R;OWENS, E;VARY, T
为了了解白细胞介素-1 （IL-1）在脓毒症诱导的骨骼肌分解代谢中的作用，我们研究了一种特异性IL-1受体拮抗剂（IL-1ra）对慢性腹部脓毒症啮齿动物模型骨骼肌蛋白代谢的影响。在脓毒症诱导后立即开始持续输注IL-1ra (2 mg.kg（-1).h(-1)）或生理盐水，持续5天。在含有不同纤维类型的单个肌肉（腓肠肌、比目鱼肌、心脏）中检测IL-1ra对蛋白质代谢的影响。在对照动物中输注IL-1ra并没有改变任何肌肉的蛋白质代谢。脓毒症降低了腓肠肌的肌肉重量、蛋白质含量和蛋白质合成率，而比目鱼肌和心脏的这些参数均未受到影响。输注IL-1ra可防止败血症引起的肌肉蛋白损失和腓肠肌蛋白合成的抑制，但对比目鱼肌和心脏没有影响。输注IL-1ra可恢复脓毒症大鼠腓肠肌的翻译效率，对RNA含量无影响。这些结果为IL-1作为败血症诱导的骨骼肌蛋白代谢异常的介质的作用提供了证据。
To understand the role of interleukin-1 (IL-1) as a mediator of the sepsis-induced skeletal muscle catabolism, we investigated the effects of a specific IL-1 receptor antagonist (IL-1ra) on skeletal muscle protein metabolism in a rodent model of chronic abdominal sepsis. A constant infusion of IL-1ra (2 mg.kg(-1).h(-1)) or saline was begun immediately after the induction of sepsis and continued for 5 days. The effect of IL-1ra on protein metabolism was examined in individual muscles (gastrocnemius, soleus, heart) containing different fiber types. Infusion of IL-1ra in control animals did not alter protein metabolism in any of the muscles examined. Muscle weight, protein content, and the rate of protein synthesis in gastrocnemius were reduced by sepsis, whereas none of these parameters were affected in soleus or heart. Infusion of IL-1ra prevented the sepsis-induced loss of muscle protein and inhibition of protein synthesis in gastrocnemius but was without effect in soleus or heart. IL-1ra infusion restored translational efficiency in the gastrocnemius of septic rats and was without effect on the RNA content. These results provide evidence for a role of IL-1 as a mediator of the sepsis-induced abnormalities in skeletal muscle protein metabolism.