Cell Cycle-Dependent Phosphorylation of MAN1
Cell Cycle-Dependent Phosphorylation of MAN1
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DOI：
10.1021/bi802060v
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发表时间：
2009-02-24
期刊：
影响因子：
2.9
通讯作者：
Takeyasu, Kunio
中科院分区：
文献类型：
--
作者：
Hirano, Yasuhiro;Iwase, Yuka;Takeyasu, Kunio
LEM（LAP 2 β、Emerin和MAN 1）蛋白是核膜通过与自整合屏障因子（BAF）结合靶向染色质所必需的。在此，我们集中在有丝分裂的磷酸化MAN 1和它的生物学作用。MAN 1在非洲爪蟾卵无细胞系统中以细胞周期依赖性方式磷酸化，并且MAN 1 N-末端区域的有丝分裂磷酸化抑制MAN 1与BAR Titansphere柱层析的结合，随后通过MS/MS测序鉴定出至少三个M期特异性磷酸化位点，Thr-209、Ser-351和Ser-402，以及一个细胞周期非依赖性磷酸化位点，463号序列突变体S402 A和S402 E的体外BAF结合试验表明，Ser-402的磷酸化对于MAN 1与BAF的结合的调节是重要的。
The LEM (LAP2 beta, Emerin, and MAN1) proteins are essential for nuclear membrane targeting to chromatin via an association with barrier-to-autointegration factor (BAF). Herein, we focused on the mitotic phosphorylation of MAN1 and its biological role. MAN1 was phosphorylated in a cell cycle-dependent manner in the Xenopus egg cell-free system, and the mitotic phosphorylation at the N-terminal region of MAN1 suppressed the binding of MAN1 to BAR Titansphere column chromatography followed by MS/MS sequencing identified at least three M-phase-specific phosphorylation sites, Thr-209, Ser-351, and Ser-402, and one cell cycle-independent phosphorylation site, Ser-463. An in vitro BAF binding assay involving mutants S402A and S402E suggested that the phosphorylation of Ser-402 was important for regulation of the binding of MAN1 to BAF.