Comprehensive analysis of the mechanisms underlying enhanced growth and root N acquisition in rice by the endophytic diazotroph, Burkholderia vietnamiensis RS1
Comprehensive analysis of the mechanisms underlying enhanced growth and root N acquisition in rice by the endophytic diazotroph, Burkholderia vietnamiensis RS1
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DOI：
10.1007/s11104-020-04506-3
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发表时间：
2020-04
期刊：
影响因子：
4.9
通讯作者：
Rina Shinjo;A. Tanaka;D. Sugiura;Takamasa Suzuki;Kazuma Uesaka;Yumiko Takebayashi;M. Kojima;H. Sakak
中科院分区：
文献类型：
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作者：
Rina Shinjo;A. Tanaka;D. Sugiura;Takamasa Suzuki;Kazuma Uesaka;Yumiko Takebayashi;M. Kojima;H. Sakak
本研究的目的是提供一个全面的了解水稻（Oryza sativaL.）通过转录组和激素单体分析，研究了B. VietnamiensisRS 1对水稻生长和根系构型的影响。鉴定影响接种B. vietnamiensisRS 1，进行转录组分析。我们还研究了激素的变化与根的形态变化的激素单体分析。B.以乙炔还原活性（ARA）为指标，测定了B. vietnamiensisRS 1的施用促进了水稻地上部和根系的生长，同时也提高了根系对氮的吸收速率，尤其是在高氮条件下。由于B.检测到ARA上的vietnamiensisRS 1。转录组分析显示，增加了与氮化合物的运输和同化相关的基因的表达，支持增强根系对氮的吸收。吲哚-3-乙酸（indole-3-acetic acid，IAA）信号转导基因的表达量增加，IAA浓度增加，这可能与根系发育加快有关。vietnamiensisRS 1通过增强根系形态发育和氮吸收能力促进氮素积累，从而促进水稻生长，这可能与在氮素供应丰富的条件下，增加氮素的运输和同化活动有关。
AimsThe aim of this study was to provide a comprehensive understanding of the mechanisms underlying response of rice (Oryza sativaL.) to the endophytic N2-fixing bacteriumBurkholderia vietnamiensisstrain RS1 in growth and nitrogen (N) accumulation using transcriptome and hormonome analyses.MethodsThe effects ofB. vietnamiensisRS1 on rice growth and root architecture were examined in pot and field conditions. To identify candidate plant genes that affect the growth of plants inoculated withB. vietnamiensisRS1, transcriptome analysis was conducted. We also examined the hormonal changes by hormonome analysis in relation to the morphological changes in roots. The contribution of N2fixation byB. vietnamiensisRS1 was estimated by the acetylene reduction activity (ARA).ResultsThe inoculation ofB. vietnamiensisRS1 resulted in increased shoot and root growth of rice plants accompanied by an increased root N absorption rate, especially under high N conditions. The contribution from N2fixation was minor because no effect ofB. vietnamiensisRS1 on ARA was detected. Transcriptome analysis revealed an increase in the expression of genes related to the transportation and assimilation of N compounds, supporting the enhanced N absorption by roots. The expression of indole-3-acetic acid (IAA) signaling genes and concentrations of IAA increased, which may be related to the increased root development.ConclusionsB. vietnamiensisRS1 enhanced rice growth by promoting N accumulation through enhanced root morphological development and N absorption ability; this outcome was probably related to the increased activities of N transportation and assimilation in the presence of a rich N supply.