Label-free Electrochemical Impedance Detection of Rotavirus Based on Immobilized Antibodies on Gold Sononanoparticles
Label-free Electrochemical Impedance Detection of Rotavirus Based on Immobilized Antibodies on Gold Sononanoparticles
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DOI：
10.1002/elan.201600179
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发表时间：
2016-08-01
期刊：
影响因子：
3
通讯作者：
Amine, Aziz
中科院分区：
文献类型：
--
作者：
Attar, Aisha;Mandli, Jihane;Amine, Aziz
目前，轮状病毒（RV）是全球严重儿童胃肠炎的主要原因。本研究提出了一种灵敏和简单的无标记测定轮状病毒，首次使用电化学阻抗谱（EIS）。半胱胺在金纳米粒子修饰玻碳电极上自组装成单分子膜，制备了免疫传感器。采用电极界面的电子传递电阻（R-et）作为检测轮状病毒的参数。该免疫传感器在4.6 ~ 4.6 × 10（4）PFUmL（-1）范围内，阻抗变化与对数值呈良好的线性关系（r（2）=0.993），检测下限为2.3PFUmL（-1），总分析时间为55 min。对免疫传感器的工作参数如pH值、抗体浓度和免疫反应时间进行了研究和优化。该传感器对甲型肝炎病毒（HAV）、肠道病毒等非靶病毒无明显干扰，具有良好的灵敏度、选择性和稳定性。
Currently, rotaviruses (RVs) are the leading cause of severe childhood gastroenteritis worldwide. This study proposes a sensitive and simple label-free assay for determining rotaviruses using, for the first time, electrochemical impedance spectroscopy (EIS). The immunosensor is fabricated by self-assembled monolayers (SAMs) of cysteamine on gold sononanoparticles (AuSNPs) modified glassy carbon electrode. The electron transfer resistance (R-et) of the electrode interface was adopted as a parameter for the detection of rotavirus. Using the proposed immunosensor, a good linear relationship (r(2)=0.993) between impedance change and logarithmic value of rotaviruses in a wide range of 4.6 to 4.6x10(4)PFUmL(-1) was found, with a low limit of detection (LOD) 2.3PFUmL(-1) and a total analysis time of 55min. Working parameters of the immunosensor, such as pH, antibodies concentration and immune-reaction time, have been investigated and optimized. No obvious interferences from non-target viruses such as hepatitis A virus (HAV) and enterovirus were found. In addition, the electrochemical impedance immunosensor has exhibited good sensitivity, selectivity and stability.