Patchy field defects of apoptosis resistance and dedifferentiation in flat mucosa of colon resections from colon cancer patients
Patchy field defects of apoptosis resistance and dedifferentiation in flat mucosa of colon resections from colon cancer patients
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DOI：
10.1007/bf02557276
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发表时间：
2002-06-01
影响因子：
3.7
通讯作者：
Bernstein, C
中科院分区：
文献类型：
--
作者：
Bernstein, H;Holubec, H;Bernstein, C
背景：外观正常的平坦结肠粘膜中的异常区域（视野缺损）可能使个体易患结肠癌。场缺陷的标记物将指示癌症风险。方法：我们评估了细胞凋亡能力，去分化，简单异常隐窝的频率，异常隐窝病灶，微腺瘤，和总烟酰胺腺嘌呤二核苷酸水平，这些水平在结肠切除术中获得的正常扁平粘膜内。结果：在来自无结肠肿瘤个体的结肠粘膜样品的杯状细胞中，在85.0%的样品中存在59.1%的高平均脱氧胆酸盐诱导的凋亡指数（AI）和高双花扁豆凝集素（DBA）凝集素反应性（分化）。相比之下，结肠癌患者的平坦粘膜样品具有显著（P <0.01）较低的平均AI 37.4%，并且显著（P = 0.03）较低的百分比（40.5%）具有高DBA反应性。对于结肠癌患者，AI和DBA反应性值在切除术内呈斑片状。烟酰胺腺嘌呤二核苷酸水平是高度可变的个体之间没有肿瘤，异常隐窝病灶和微adenomas是罕见的。结论：在和异常DBA反应是有前途的指标结肠癌的风险。我们的研究结果证明了获得多个样本来评估结肠癌风险的重要性，因为视野缺损的斑块性质。
Background: Abnormal areas in normal-appearing flat colonic mucosa (field defects) may predispose individuals to colon cancer. Markers of field defects would indicate cancer risk.Methods: We evaluated apoptosis capability, dedifferentiation, frequency of simple aberrant crypts, aberrant crypt foci, microadenomas, and total nicotinamide adenine dinucleotide levels at locations within normal-appearing flat mucosa obtained from colon resections.Results: Among goblet cells from colonic mucosa samples of individuals without colonic neoplasia, there was a high mean deoxycholate-induced apoptotic index (AI) of 59.1% and high Dolichos biflorus agglutinin (DBA) lectin reactivity (differentiation) in 85.0% of samples. In contrast, flat mucosa samples from colon cancer patients had a significantly (P < .01) lower average AI of 37.4%, and a significantly (P = .03) lower percentage (40.5%) had high DBA reactivity. For colon cancer patients, AI and DBA reactivity values were patchy within a resection. Nicotinamide adenine dinucleotide levels were highly variable among individuals without neoplasia, and aberrant crypt foci and microadenomas were rare.Conclusions: At and aberrant DBA reactivity are promising indicators of colon cancer risk. Our results attest to the importance of obtaining multiple samples to assess colon cancer risk because of the patchy nature of field defects.