A putative leucine-rich repeat receptor kinase, OsBRR1, is involved in rice blast resistance
A putative leucine-rich repeat receptor kinase, OsBRR1, is involved in rice blast resistance
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DOI：
10.1007/s00425-009-0951-1
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发表时间：
2009-07-01
期刊：
影响因子：
4.3
通讯作者：
Lu, Tiegang
中科院分区：
文献类型：
--
作者：
Peng, Hao;Zhang, Qian;Lu, Tiegang
富含亮氨酸重复序列受体样激酶（Leucine-rich repeat receptor-like kinases，LRR-RLKs）是植物中最大的跨膜受体样激酶亚家族，参与植物的发育和防御过程。本研究利用RNA干扰（RNAi）技术特异性地敲除了59个水稻LRR-RLKs基因，并利用稻瘟病菌弱毒力菌株Ken 54-04筛选T-0 RNAi群体，获得了一个新的水稻抗稻瘟病相关基因OsBRR 1。野生型植物（Oryza sativa L.）CV.‘日本晴’）对Ken 54-04表现出中等抗性，而OsBRR 1抑制植株对Ken 54-04感病。此外，OsBRR 1过表达植株对一些致病菌株（97-27-2、99-31-1和中10-8-14）的抗性增强。OsBRR 1在正常生长条件下，在叶片中表达量较低，在根中检测不到，而在感染稻瘟病菌（Ken 54-04）的叶片中，OsBRR 1的转录被显著诱导，阿坝、JA和SA处理对OsBRR 1的表达有一定的影响。OsBRR 1的过表达或RNAi抑制不会引起水稻植株明显的发育变化。这些结果表明OsBRR 1参与了水稻对稻瘟病的抗性反应，并介导了水稻对稻瘟病的抗性。
Leucine-rich repeat receptor-like kinases (LRR-RLKs) comprise the largest subfamily of transmembrane receptor-like kinases in plants, and they regulate a wide variety of developmental and defense-related processes. In this study, RNA interference (RNAi) strategy was used to specifically knockdown 59 individual rice genes encoding putative LRR-RLKs, and a novel rice blast resistance-related gene (designated as OsBRR1) was identified by screening T-0 RNAi population using a weakly virulent isolate of Magnaporthe oryzae, Ken 54-04. Wild-type plants (Oryza sativa L. cv. 'Nipponbare') showed intermediate resistance to Ken 54-04, while OsBRR1 suppression plants were susceptible to Ken 54-04. Furthermore, OsBRR1-overexpressing plants exhibited enhanced resistance to some virulent isolates (97-27-2, 99-31-1 and zhong 10-8-14). OsBRR1 expression was low in leaves and undetectable in roots under normal growth conditions, while its transcript was significantly induced in leaves infected with the blast fungus (Ken 54-04) and was moderately affected by ABA, JA and SA treatment. Overexpression or RNAi suppression of OsBRR1 did not cause visible developmental changes in rice plants. These results indicate that OsBRR1 is involved in rice resistance responses to blast fungus and mediates resistance to rice blast.