Houttuynia cordata modulates oral innate immune mediators: potential role of herbal plant on oral health.
Houttuynia cordata modulates oral innate immune mediators: potential role of herbal plant on oral health.
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DOI：
10.1111/odi.12313
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发表时间：
2015-05
期刊：
影响因子：
3.8
通讯作者：
S. Satthakarn;W. Chung;A. Promsong;Wipawee Nittayananta
中科院分区：
文献类型：
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作者：
S. Satthakarn;W. Chung;A. Promsong;Wipawee Nittayananta
目的上皮细胞通过产生多种免疫介质在口腔先天免疫中发挥积极作用。鱼腥草 (H. cordata) 是一种发现于亚洲的草本植物，具有多种活性。然而，其对口腔先天免疫的影响从未有报道。本研究的目的是确定 H. cordata 提取物对口腔上皮细胞产生的先天免疫介质表达的影响。材料和方法 用不同浓度的提取物处理原代牙龈上皮细胞 (GEC) 18 小时。使用定量实时 RT-PCR 测量 hBD2、SLPI、细胞因子和趋化因子的基因表达。通过ELISA或Luminex测定检测培养上清液中的分泌蛋白。使用 CellTiter-Blue Assay 评估提取物的细胞毒性。结果 H. cordata 以剂量依赖性方式显着诱导 hBD2、SLPI、IL-8 和 CCL20 的表达，且没有细胞毒性。提取物调节分泌的 hBD2 和 SLPI 蛋白，并显着诱导 IL-2、IL-6、IL-8 和 IFN-γ 的水平。结论 我们的数据表明 H. cordata 可以调节口腔先天免疫介质。这些发现可能有助于开发来自 H. cordata 的新外用制剂，用于预防和治疗免疫介导的口腔疾病。
OBJECTIVE Epithelial cells play an active role in oral innate immunity by producing various immune mediators. Houttuynia cordata Thunb (H. cordata), a herbal plant found in Asia, possesses many activities. However, its impacts on oral innate immunity have never been reported. The aim of this study was to determine the effects of H. cordata extract on the expression of innate immune mediators produced by oral epithelial cells. MATERIALS AND METHODS Primary gingival epithelial cells (GECs) were treated with various concentrations of the extract for 18 h. The gene expression of hBD2, SLPI, cytokines, and chemokines was measured using quantitative real-time RT-PCR. The secreted proteins in the culture supernatants were detected by ELISA or Luminex assay. Cytotoxicity of the extract was assessed using CellTiter-Blue Assay. RESULTS H. cordata significantly induced the expression of hBD2, SLPI, IL-8, and CCL20 in a dose-dependent manner without cytotoxicity. The secreted hBD2 and SLPI proteins were modulated, and the levels of IL-2, IL-6, IL-8, and IFN-γ were significantly induced by the extract. CONCLUSIONS Our data indicated that H. cordata can modulate oral innate immune mediators. These findings may lead to the development of new topical agents from H. cordata for the prevention and treatment of immune-mediated oral diseases.