Restricted feeding schedules phase shift daily rhythms of c-Fos and protein Per1 immunoreactivity in corticolimbic regions in rats
Restricted feeding schedules phase shift daily rhythms of c-Fos and protein Per1 immunoreactivity in corticolimbic regions in rats
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DOI：
10.1016/j.neuroscience.2006.08.064
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发表时间：
2007-01-05
期刊：
影响因子：
3.3
通讯作者：
Escobar, C.
中科院分区：
文献类型：
--
作者：
Angeles-Castellanos, M.;Mendoza, J.;Escobar, C.
在每日限制进食时间表(RFS)的夹带作用下，动物产生食物预期活动(FAA)，该活动涉及可能受皮质边缘结构和伏隔核调控的动机过程。本研究旨在探讨皮质边缘结构是否参与FAA的表达，因此采用c-Fos免疫反应(Fos-IR)作为神经元活性的标志物。第二个目标是确定皮质边缘结构中时钟蛋白Perl(Peri)的昼夜节律，并确定RFS对昼夜时间模式的影响。大鼠每天在RFS下进食2 h，对照组给予随意喂养。在FAA期间和用餐后，食物夹带在伏核(ACC)、基底外侧核和中央杏仁核、终纹床核(BNST)、外侧隔核(LS)、前额叶皮质(PFC)和丘脑室旁核(PVT)的Fos-IR升高。在自由活动条件下，除杏仁核和PFC外，所有被研究的结构都显示出PER1的每日振荡。RFS移动并设定伏隔、海马区、外侧隔区和PFC两个亚区的ZT时间(ZT)12的日峰值。RFS使BNST的ZT12波幅增加，PVT波峰向ZT6方向移动。杏仁核未见明显变化。目前的数据表明，皮质边缘结构的细胞激活与与食物预期活动相关的行为事件有关，进餐时间是改变皮质边缘结构中PER1每日振荡的相关信号。数据表明，皮质边缘结构作为FAA的振荡器具有相关作用。(C)2006年IBRO。爱思唯尔有限公司出版。保留所有权利。
Entrainment by daily restricted feeding schedules (RFS) produces food anticipatory activity (FAA) which involves motivational processes which may be regulated by corticolimbic structures and the nucleus accumbens. The present study aimed first to determine whether corticolimbic structures participate in the expression of FAA, therefore c-Fos immunoreactivity (Fos-IR) was employed as marker of neuronal activity. The second goal was to characterize diurnal rhythms of the clock protein protein Perl (PERI) in corticolimbic structures and to determine the influence of RFS on the diurnal temporal pattern.Rats were maintained under RFS with food access for 2 h daily, a control group was fed ad libitum. Food entrainment produced a pattern of increased Fos-IR during FAA and after mealtime in the two sub-regions of the nucleus accumbens (ACC), in the basolateral and central amygdala, in the bed nucleus of the stria terminalis (BNST), in the lateral septum (LS), in the prefrontal cortex (PFC), and in the paraventricular thalamic nucleus (PVT). No increased Fos-IR was observed in the hippocampus.Under ad libitum conditions all structures studied showed daily oscillations of PER1, excluding both amygdalar nuclei and the PFC. RFS shifted and set the daily peaks at zeitgeber time (ZT) 12 for both sub-regions in the accumbens, the hippocampus, lateral septum and PFC. RFS enhanced the amplitude at ZT12 of the BNST and shifted the peak of the PVT to ZT6. No changes were observed in the amygdalar nuclei. Present data indicate that cellular activation of corticolimbic structures is associated with behavioral events related to food anticipatory activity and that mealtime is a relevant signal that shifts daily oscillations of PER1 in corticolimbic structures. Data suggest a relevant role of corticolimbic structures as oscillators for FAA. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.