CHANGES IN PERMEABILITY OF STAPHYLOCOCCUS-AUREUS AND DERIVED LIPOSOMES WITH VARYING LIPID COMPOSITION
CHANGES IN PERMEABILITY OF STAPHYLOCOCCUS-AUREUS AND DERIVED LIPOSOMES WITH VARYING LIPID COMPOSITION
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DOI：
10.1016/0005-2736(72)90385-9
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发表时间：
1972-01-01
期刊：
影响因子：
--
通讯作者：
OPDENKAM.JA
中科院分区：
文献类型：
--
作者：
HAEST, CWM;VANDEENE.LL;OPDENKAM.JA
在膜模型系统中研究了磷脂磷脂酰甘油和赖氨酰磷脂酰甘油的物理和阻隔性能。在空气-水界面处的单层堆积表明，赖氨酰磷脂酰甘油的每个分子面积比磷脂酰甘油更大。赖氨酰磷脂酰甘油制备的脂质体的非电解质渗透性高于磷脂酰甘油制备的脂质体。另一方面，磷脂酰甘油的脂质体的86Rb+的渗透性高于赖氨酰磷脂酰甘油的脂质体。缬氨霉素只能增加磷脂酰甘油脂质体中该阳离子的通透性。环境pH值对金黄色葡萄球菌完整细胞中赖氨酰磷脂酰甘油与磷脂酰甘油比例影响的研究表明，赖氨酰磷脂酰甘油、心磷脂和中性甘油的总量 当培养基的pH值在6.5和5.0之间变化时，膜中的脂质没有变化；但当培养基pH值降低时，磷脂酰甘油总量减少。完整细胞对赤藓糖醇的渗透性似乎随着赖氨酰磷脂酰甘油与磷脂酰甘油比率的增加而增加；而当该比率增加时，缬氨霉素介导的86Rb+在细胞膜上的交换似乎减少。从细胞和脂质体的渗透性特性之间的相关性得出结论，在S中。金黄色葡萄球菌磷脂的化学性质在很大程度上决定了渗透性屏障的性质。
The physical and barrier properties of the phospholipids phosphatidylglycerol and lysylphosphatidylglycerol were studied in membrane model systems. Packing in monolayers at the air-water interface showed a larger area per molecule for lysylphosphatidylglycerol than for phosphatidylglycerol. The non-electrolyte permeability of liposomes prepared with lysylphosphatidylglycerol was higher than of those prepared with phosphatidylglycerol. On the other hand, the permeability of86Rb+was higher for liposomes of phosphatidylglycerol than for those of lysylphosphatidylglycerol. Valinomycin was able to increase the permeability of this cation in the phosphatidylglycerol liposome only.Studies on the effect of the environmental pH on the lysylphosphatidylglycerol to phosphatidylglycerol ratio in intact cells ofStaphylococcus aureusshowed that the total amount of lysylphosphatidylglycerol, cardiolipin and neutral lipids in the membrane did not change when the pH of the medium was varied between pH 6.5 and 5.0; but the total amount of phosphatidylglycerol decreased when the pH of the medium was lowered. The permeability of the intact cells for erythritol appeared to increase with increasing lysylphosphatidylglycerol to phosphatidylglycerol ratio; whereas the valinomycin mediated exchange of86Rb+over the cell membrane appeared to decrease when this ratio was increased.From the correlation between the permeability properties of cells and liposomes the conclusion is drawn that inS. aureusthe chemical nature of the phospholipids determines to a great extent the properties of the permeability barrier.