Placental infection with human papillomavirus is associated with spontaneous preterm delivery
Placental infection with human papillomavirus is associated with spontaneous preterm delivery
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DOI：
10.1093/humrep/dem404
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发表时间：
2008-03-01
影响因子：
6.1
通讯作者：
Parry, S.
中科院分区：
文献类型：
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作者：
Gomez, L. M.;Ma, Y.;Parry, S.
背景：我们试图确定人类乳头瘤病毒(HPV)感染绒毛外滋养层细胞是否减少细胞侵袭，以及胎盘感染是否与胎盘功能障碍所致的不良生殖结局有关。方法：我们用绒毛外滋养层细胞(HTR-8/SVneo)进行了细胞凋亡和侵袭实验，该细胞被含有HPV-16全基因组的质粒(PAT-HPV-16)感染。为了将HPV感染与生殖结局联系起来，我们进行了一项病例对照研究，检测了自发性早产、37周需要分娩的重度子痫前期和足月分娩的对照组胎盘绒毛外滋养细胞区域的HPV DNA。结果：转染组细胞凋亡率是未转染组或空质粒转染组的3~6倍。细胞外基质对转基因细胞的侵袭能力比对照组低25-58%。自然早产组胎盘组织中HPV阳性率明显高于对照组(P=0.03)。子痫前期患者胎盘组织中HPV的检测结果与对照组无显著差异。结论：HPV感染绒毛外滋养细胞可诱导细胞死亡，可能减少胎盘对宫壁的侵袭。因此，HPV感染可能导致胎盘功能障碍，并与不良妊娠结局有关，包括自发早产。
BACKGROUND: We sought to determine if human papillomavirus (HPV) infection of extravillous trophoblast cells reduces cell invasion and if placental infection is associated with adverse reproductive outcomes attributed to placental dysfunction. METHODS: We conducted apoptosis and invasion assays using extravillous trophoblast (HTR-8/SVneo) cells that were transfected with a plasmid (pAT-HPV-16) containing the entire HPV-16 genome. In order to associate HPV infection with reproductive outcomes, we conducted a case-control study to detect HPV DNA in the extravillous trophoblast region of placentas from cases of spontaneous preterm delivery, severe pre-eclampsia requiring delivery at < 37 weeks and controls who delivered at term. RESULTS: Rates of apoptosis were 3- to 6-fold greater in transfected cells than in non-transfected cells or cells transfected with an empty plasmid. Invasion of transfected cells through extracellular matrices was 25-58% lower than that of the controls. HPV was detected more frequently in placentas from spontaneous preterm deliveries than in placentas from controls (P = 0.03). Identification of HPV in placentas from cases of pre-eclampsia was not significantly different to controls. CONCLUSIONS: HPV infection of extravillous trophoblast induces cell death and may reduce placental invasion into the uterine wall. Thus, HPV infection may cause placental dysfunction and is associated with adverse pregnancy outcomes, including spontaneous preterm delivery.