Evidence of reactive oxygen species generation in synovial fluid from patients with temporomandibular disease by electron spin resonance spectroscopy
Evidence of reactive oxygen species generation in synovial fluid from patients with temporomandibular disease by electron spin resonance spectroscopy
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DOI：
10.1179/135100004225006830
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发表时间：
2004-01-01
期刊：
影响因子：
3.8
通讯作者：
Kubota, E
中科院分区：
文献类型：
--
作者：
Lee, MC;Kawai, Y;Kubota, E
活性氧（ROS）与颞下颌疾病的发病机制有关。在本研究中，我们通过电子自旋共振（ESR）和自旋捕获提供了人类颞下颌疾病患者滑液中ROS生成的第一个证据。在滑液中观察到三种不同的DMPO自旋加合物的ESR谱。它们对应于三种自由基：羟基自由基（HO.）、氢自由基（H-.）和碳中心自由基（R-.）。其中，5,5-二甲基-1-吡咯啉- n -氧化物(DMPO)-OH光谱最为突出，表明HO。主要产生于颞下颌紊乱患者的滑液中。去铁胺（DFO）是一种铁螯合剂，可强烈抑制颞下颌关节疾病患者滑液中的dpo - oh信号强度。我们成功地证明了ros诱导的颞下颌疾病患者滑液中的氧化应激。颞下颌关节中ROS的产生可导致炎症加剧和软骨基质降解酶的激活，从而导致颞下颌关节退行性改变。因此，铁依赖性HO的产生。可能在颞下颌紊乱的发病机制中起关键作用。
Reactive oxygen species (ROS) have been implicated in the pathogenesis of temporomandibular disorders. In the present study, we provide the first evidence of ROS generation in the synovial fluid from human temporomandibular disorder patients, as shown by electron spin resonance (ESR) and spin trapping. Three distinct ESR spectra of DMPO spin adducts were observed in the synovial fluid. They corresponded to three free radical species: hydroxyl radical (HO.), hydrogen radical (H-.), and carbon-center radical (R-.). Among them, the 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)-OH spectrum was the most prominent, suggesting that HO. was dominantly generated in the synovial fluid from temporomandibular disorder patients. Desferrioxamine (DFO), an iron chelator, strongly depressed the DMPO-OH signal intensity in the synovial fluid from patients with temporomandibular disorders. We successfully demonstrated ROS-induced oxidative stress in the synovial fluid from temporomandibular disorder patients. ROS generation in the temporomandibular joint could lead to exacerbation of inflammation and activation of cartilage matrix degrading enzymes that proceed to degenerative change of the temporomandibular joint. Thus, iron-dependent generation of HO. might have a crucial role in the pathogenesis of temporomandibular disorders.