MATING SIGNALS CONTROL EXPRESSION OF MUTATIONS RESULTING FROM INSERTION OF A TRANSPOSABLE REPETITIVE ELEMENT ADJACENT TO DIVERSE YEAST GENES
MATING SIGNALS CONTROL EXPRESSION OF MUTATIONS RESULTING FROM INSERTION OF A TRANSPOSABLE REPETITIVE ELEMENT ADJACENT TO DIVERSE YEAST GENES
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DOI：
10.1016/0092-8674(80)90353-0
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
WIAME, JM
中科院分区：
文献类型：
--
作者：
ERREDE, B;CARDILLO, TS;WIAME, JM
ROAM（响应交配信号的调节过度生产等位基因）突变导致酿酒酵母过度生产。 MATa/MAT.alpha 中 ROAM 突变基因产物的过量产生较少。不能缀合的二倍体菌株比可以缀合的单倍体菌株。过量生产发生在能够交配的二倍体菌株中，无论它们是否能够形成孢子。当单倍体 ROAM 突变体还含有阻止接合的 ste7 突变时，过量生产就会减少；其他ste突变不影响ROAM突变的表达。 ROAM突变基因CYC7-H2的克隆表明，与可转座和重复的Ty1元件同源的5.5kb[千碱基]序列被插入到CYC7结构位点的5''非编码区中。其他 ROAM 突变的相似遗传特性表明它们均包含插入的 Ty 元件。这些结果还表明，突变对通常针对控制缀合功能的基因的信号作出反应，并且Ty元件中存在的序列可能与结构位点相邻，并且是这些信号的正常受体。
ROAM [regulated overproducing alleles responding to mating signals] mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT.alpha. diploid strains which cannot conjugate than in haploid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproduction decreases when haploid ROAM mutants also contain the ste7 mutation which prevents conjugation; other ste mutations do not affect the expression of ROAM mutations. Cloning of the ROAM mutant gene CYC7-H2 shows that a 5.5 kb [kilobase] sequence homologous to a transposable and reiterated Ty1 element is inserted in the 5'' noncoding region of the CYC7 structural locus. The similar genetic properties of other ROAM mutations suggest that they each contain an inserted Ty element. These results also suggest that mutations respond to signals normally directed toward genes controlling conjugation functions, and that sequences present in Ty elements may be adjacent to structural loci and are the normal receptors for these signals.