CLONING AND MAPPING OF THE SPO1 GENOME
CLONING AND MAPPING OF THE SPO1 GENOME
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DOI：
10.1016/0042-6822(85)90424-6
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
STEWART, CR
中科院分区：
文献类型：
--
作者：
CURRAN, JF;STEWART, CR
噬菌体SPO1的许多Xba I、EcoRI*、Kpm I和Bgl II片段，占65%的基因组序列在枯草芽孢杆菌中被克隆。其中4个EcoRI* 片段在大肠杆菌和B中都是特异性难克隆的。枯草芽孢杆菌，可能是因为表达的有害基因携带的SPO1片段。为了能够完全鉴定克隆的区域，SPO1限制性酶切图谱已扩展到包括XbaI片段和以前未定位的Kpn I片段。39个已知基因中有26个的标记位于特定的克隆片段上，从而可以更精确地确定大多数基因的位置。克隆的一个片段对SPO1的发育有抑制作用。
Many of the XbaI, EcoRI*, KpmI anmd BglII fragments of bacteriophage SPO1, accounting for .apprx. 65% of the genomic sequences, were cloned in Bacillus subtilis. Four of the EcoRI* fragments were specifically refractory to cloning in both Escherichia coli and B. subtilis, probably because of expression of deleterious genes carried on the SPO1 fragments. To permit complete identification of the regions cloned, the SPO1 restriction map has been extended to include the XbaI fragments and the previously unmapped KpnI fragments. Markers for 26 of the 39 known genes were located on specific cloned fragments, permitting more precise determination of the positions of most of the genes. One cloned SPO1 fragment was inhibitory to SPO1 development.