Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei
Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei
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DOI：
10.1016/j.molbiopara.2007.02.008
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发表时间：
2007-06-01
影响因子：
1.5
通讯作者：
Roditi, Isabel
中科院分区：
文献类型：
--
作者：
Burkard, Gabriela;Fragoso, Cristina M.;Roditi, Isabel
本文描述了Nucleofector系统在布氏锥虫血流和前环形式瞬时和稳定转染中的应用，以及与标准电穿孔方案的比较。该系统基于预先设定的程序和未知成分的“细胞类型特异性”溶液的组合，允许转染的DNA直接进入细胞核，而不需要细胞分裂。它是1000倍以上的效率为T的稳定转化。brucei血流形式比常规电穿孔，并且与用前循环形式获得的速率相当。
This paper describes the application of the Nucleofector system for transient and stable transfection of bloodstream and procyclic forms ofTrypanosoma brucei, and its comparison with standard electroporation protocols. The system, which is based on a combination of pre-set programmes and "cell-type specific" solutions of unknown composition, allows transfected DNA to enter the nucleus directly without the need for cell division. It is 1000-fold more efficient for stable transformation ofT. bruceibloodstream forms than conventional electroporation, and is comparable to the rate obtained with procyclic forms.