Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in New York
Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in New York
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DOI：
10.4269/ajtmh.2006.75.1140
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发表时间：
2006-12-01
影响因子：
3.3
通讯作者：
Howard, John J.
中科院分区：
文献类型：
--
作者：
Molaei, Goudarz;Oliver, Joanne;Howard, John J.
东部马脑炎（EAE）病毒在涉及嗜鸟蚊子载体（主要是Culiseta melanura（Coquillett）和禽类扩增宿主）的地方流行周期中持续存在。为了更好地理解C的作用。在2004年5月31日至10月15日期间，我们在纽约中部与埃博拉病毒疫源地相关的两个地点收集了吸血蚊子，并通过细胞色素B基因的聚合酶链反应（PCR）产物的核苷酸测序鉴定了脊椎动物血液的来源。484 Cs的分析。melanura和122 Cs.其中94.2%和86.9%的感染者仅从禽类宿主获得血液。在0.8%的Cs中检测到完全来自哺乳动物的血粉。melanura和1.6%的Cs。Morsitans。个别蚊子含有混合血液的膳食从鸟类和哺乳动物的主机也检测到5.0%的Cs。melanura和11.5%的Cs。Morsitans。林鸫构成了最常见的脊椎动物主机的Cs。melanura（23.6%）和Cs.其次是美洲知更鸟、歌雀、灶嘴鸟、红眼绿鹃和黄喉鸫。哺乳动物来源的血粉被确定为白尾鹿，马，家猫，和东部伏翼蝙蝠。从Cs中分离到3株病毒。melanura和一个来自Cs。Morsitans。这些结果表明，林鸫和其他一些雀形目鸟类可能发挥关键作用，在东北部，并可能在整个地理范围内的传播，在北美。哺乳动物进食的频率也表明Cs。melanura和Cs.除了在禽类宿主之间维持地方性传播外，莫氏杆菌可能在将马疱疹病毒传播给马方面发挥作用。我们首次从蚊媒中分离出虫媒病毒，并鉴定了其血餐来源。
Eastern equine encephalitis (EEE) virus perpetuates in an enzootic cycle involving ornithophilic mosquito vectors, principally Culiseta melanura (Coquillett) and avian amplification hosts. To better understand the role of Cs. melanura and Culiseta morsitans (Theobald) in the epizootiology of EEE virus, we collected blood-fed mosquitoes between 31 May and 15 October 2004 at two sites associated with an EEE virus focus in central New York and identified the source of vertebrate blood by nucleotide sequencing of polymerase chain reaction (PCR) products of the cytochrome b gene. Analysis of 484 Cs. melanura and 122 Cs. morsitans revealed that 94.2% and 86.9%, respectively, acquired blood solely from avian hosts. Blood meals derived exclusively from mammals were detected in 0.8% of Cs. melanura and 1.6% of Cs. morsitans. Individual mosquitoes containing mixed-blood meals from both avian and mammalian hosts were also detected in 5.0% of Cs. melanura and 11.5% of Cs. morsitans. Wood thrush constituted the most common vertebrate host for Cs. melanura (23.6%) and Cs. morsitans (30.9%), followed by American robin, song sparrow, ovenbird, red-eyed vireo, and common yellowthroat. Mammalian-derived blood meals were identified as white-tailed deer, horse, domestic cat, and eastern pipistrelle bat. There were three isolations of EEE virus from Cs. melanura and one from Cs. morsitans. These results suggest that wood thrush and a few other passerine birds may play key roles in supporting EEE virus transmission in the northeast and possibly throughout the geographic range of EEE in North America. The frequency of mammalian feedings also suggests that Cs. melanura and Cs. morsitans may play a role in the transmission of EEE virus to equines, in addition to maintaining enzootic transmission among avian hosts. We report the first isolation of arboviruses from mosquito vectors concomitant with the identifications of their blood meal sources.