Differential regulation of proteins and phosphoproteins in rice under drought stress
Differential regulation of proteins and phosphoproteins in rice under drought stress
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DOI：
10.1016/j.bbrc.2008.12.067
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发表时间：
2009-01-30
影响因子：
3.1
通讯作者：
Li, Jiaxu
中科院分区：
文献类型：
--
作者：
Ke, Yuqin;Han, Guoqiang;Li, Jiaxu
干旱是亚洲稻米生产的最大制约因素。蛋白质磷酸化已被认为是环境胁迫信号传导的重要机制。然而，干旱水稻诱导的蛋白质和磷蛋白的差异表达在很大程度上仍然未知。本文报道了利用蛋白质组学方法鉴定干旱诱导水稻差异表达的蛋白质和磷蛋白。鉴定了三种干旱响应蛋白。干旱处理上调了类莱亚蛋白和叶绿体Cu-Zn超氧化物歧化酶（SOD）的表达，下调了Rieske Fe-S前体蛋白的表达。鉴定了10种干旱响应磷蛋白：NAD-苹果酸脱氢酶、OSJNBa0084K20.14蛋白、脱落酸和胁迫诱导蛋白、核糖体蛋白、干旱诱导S样核糖核酸酶、乙烯诱导蛋白、鸟嘌呤核苷酸结合蛋白β亚基样蛋白、r-40 c1蛋白、OSJNBb0039L24.13蛋白和萌发样蛋白1。这些磷蛋白中有7种以前没有报道过参与水稻干旱胁迫。这些结果为干旱诱导蛋白的调控机制提供了新的见解，并涉及一些以前未被识别的蛋白质响应干旱胁迫。(C)2008年爱思唯尔公司All rights reserved.
Drought is the largest constraint on rice production in Asia. Protein phosphorylation has been recognized as an important mechanism for environmental stress signaling. However, the differential expression of proteins and phosphoproteins induced by drought ill rice is still largely unknown. In this paper, we report the identification of differentially expressed proteins and phosphoproteins induced by drought in rice using protemnic approaches. Three drought-responsive proteins were identified. Late embryogenesis abundant (LEA)-like protein and chloroplast Cu-Zn superoxide dismutase (SOD) were up-regulated by drought whereas Rieske Fe-S precursor protein was down-regulated. Ten drought-responsive phosphoproteins were identified: NAD-malate dehydrogenase, OSJNBa0084K20.14 protein, abscisic acid- and stress-inducible protein, ribosomal protein, drought-induced S-like ribonuclease, ethylene-inducible protein, guanine nucleotide-binding protein beta subunit-like protein, r-40c1 protein, OSJNBb0039L24.13 protein and germin-like protein 1. Seven of these phosphoproteins have not previously been reported to be involved in rice drought stress. These results provide new insight into the regulatory mechanism of drought-induced proteins and implicate several previously unrecognized proteins in response to drought stress. (C) 2008 Elsevier Inc. All rights reserved.