Optimising the storage and extraction of chlorophyll samples
Optimising the storage and extraction of chlorophyll samples
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发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
N. Wasmund;I. Topp;D. Schories
中科院分区：
文献类型：
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作者：
N. Wasmund;I. Topp;D. Schories
使用藻类培养物和波罗的海海水测试了不同方法步骤对叶绿素 (chla) 分析的影响。使用方差分析对选定的实验进行了测试，以了解处理之间的显着差异。实验结果得出以下建议：样品体积应较小，以便过滤时间不超过 10 分钟。如果需要在 -20°C 下储存样品，则应储存提取物而不是过滤器。但是，建议在液氮中快速冷冻。 96% 乙醇的提取效率比 90% 丙酮的提取效率高得多 - 在后一种溶剂中提取需要对过滤器进行均质化。如果使用推荐的96%乙醇，提取时间在3至24小时范围内没有显着影响。
The effect of different methodological steps on the analysis of chlorophylla (chla) was tested with algae cultures and Baltic Sea water. Selected experiments were tested with ANOVAs for significant differences between treatments. The results of the experiments led to the following recommendations: the sample volume should be low so that filtration takes no longer than 10 min. Extracts rather than filters should be stored if storage of the samples at −20 ◦ C is required. However, quickfreezing in liquid nitrogen is recommended. The extraction efficiency was much better in 96% ethanol than in 90% acetone – extraction in the latter solvent requires the filters to be homogenised. The extraction time has no significant influence within a range of 3 to 24 hours if the recommended 96% ethanol is used.