Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria
Final steps in juvenile hormone biosynthesis in the desert locust, Schistocerca gregaria
复制标题
DOI：
10.1016/j.ibmb.2010.12.007
复制
发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Vanden Broeck, Jozef
中科院分区：
文献类型：
--
作者：
Marchal, Elisabeth;Zhang, JinRui;Vanden Broeck, Jozef
两个基因编码酶以前报道参与保幼激素（JH）生物合成的最后步骤在不同的昆虫物种，其特征在于在沙漠蝗虫，Schistocerca gregaria。保幼激素酸O-甲基转移酶（JHAMT）先前被描述为催化法呢酸（FA）和JH酸分别转化为其甲酯法呢酸甲酯（MF）和JH。据报道，第二个基因CYP 15 A1编码细胞色素P450酶，负责将MF环氧化为JH。此外，第三个基因，FAMeT（最初报道编码法呢酸甲基转移酶）也包括在这项研究中。使用q-RT-PCR，所有三个基因（JHAMT. CYP 15 A1和FAMeT）主要在沙漠蝗虫的CA中表达，这是JH的主要生物合成组织。利用RNA干扰技术验证了这些基因在S. gregaria。在成年动物中敲除这三个基因，然后进行JH生物合成和释放的放射化学测定（RCA），表明SgJHAMT和SgCYP 15 A1分别负责MF和JH的合成。我们的实验没有显示SgFAMeT参与沙漠蝗虫JH的生物合成。SgJHAMT和SgCYP 15 A1的有效和选择性抑制剂可能代表选择性生物理性蝗虫控制剂。(C)2010爱思唯尔有限公司保留所有权利。
Two genes coding for enzymes previously reported to be involved in the final steps of juvenile hormone (JH) biosynthesis in different insect species, were characterised in the desert locust, Schistocerca gregaria. Juvenile hormone acid O-methyltransferase (JHAMT) was previously described to catalyse the conversion of farnesoic acid (FA) and JH acid to their methyl esters, methyl farnesoate (MF) and JH respectively. A second gene, CYP15A1 was reported to encode a cytochrome P450 enzyme responsible for the epoxidation of MF to JH. Additionally, a third gene, FAMeT (originally reported to encode a farnesoic acid methyltransferase) was included in this study. Using q-RT-PCR, all three genes (JHAMT. CYP15A1 and FAMeT) were found to be primarily expressed in the CA of the desert locust, the main biosynthetic tissue of JH. An RNA interference approach was used to verify the orthologous function of these genes in S. gregaria. Knockdown of the three genes in adult animals followed by the radiochemical assay (RCA) for JH biosynthesis and release showed that SgJHAMT and SgCYP15A1 are responsible for synthesis of MF and JH respectively. Our experiments did not show any involvement of SgFAMeT in JH biosynthesis in the desert locust. Effective and selective inhibitors of SgJHAMT and SgCYP15A1 would likely represent selective biorational locust control agents. (C) 2010 Elsevier Ltd. All rights reserved.