Rapid improvement of domestication traits in an orphan crop by genome editing
Rapid improvement of domestication traits in an orphan crop by genome editing
复制标题
DOI：
10.1038/s41477-018-0259-x
复制
发表时间：
2018-10-01
期刊：
影响因子：
18
通讯作者：
Lippman, Zachary B.
中科院分区：
文献类型：
--
作者：
Lemmon, Zachary H.;Reem, Nathan T.;Lippman, Zachary B.
基因组编辑对于提高作物生产力具有巨大的希望，人们对推进孤儿作物的育种特别感兴趣，孤儿作物通常具有类似于野生近缘种的不良特征。我们开发了孤儿茄科作物“地樱桃”（酸浆）的基因组资源和有效转化，并使用成簇的规则间隔短回文重复序列（CRISPR）-CRISPR相关蛋白-9核酸酶（Cas9）（CRISPR-Cas9）来突变番茄驯化和改良基因的直系同源物，这些基因控制植物结构、花产量和果实大小，从而改善这些主要生产力性状。因此，从模式作物中转化知识能够在远亲孤儿作物中快速创建目标等位基因多样性和新的育种种质。
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan Solanaceae crop 'groundcherry' (Physalis pruinosa) and used clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) (CRISPR-Cas9) to mutate orthologues of tomato domestication and improvement genes that control plant architecture, flower production and fruit size, thereby improving these major productivity traits. Thus, translating knowledge from model crops enables rapid creation of targeted allelic diversity and novel breeding germplasm in distantly related orphan crops.