Detection of tetanus-induced effects in linearly lined-up micropatterned neuronal networks: Application of a multi-electrode array chip combined with agarose microstructures
Detection of tetanus-induced effects in linearly lined-up micropatterned neuronal networks: Application of a multi-electrode array chip combined with agarose microstructures
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DOI：
10.1016/j.bbrc.2007.03.006
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发表时间：
2007-05-04
影响因子：
3.1
通讯作者：
Yasuda, Kenji
中科院分区：
文献类型：
--
作者：
Suzuki, Ikurou;Yasuda, Kenji
理解信息获取和存储机制的最佳方法之一是通过监测和刺激拓扑定义网络中的单个神经元并持续很长一段时间来表征网络活动的可塑性。因此，我们以前开发了一个芯片上的多电极阵列（MEA）系统与琼脂糖微室（AM，Cs）的阵列相结合。可以长期同时记录多个细胞的放电，并在形貌上控制细胞的位置及其连接。在我们目前的研究中，我们证明了强直刺激在AMC/MEA芯片上线性排列的图案化网络中的效果。我们检测到由强直刺激诱导的可再现的活性变化，并看到这些变化维持6-24小时。结果表明，我们的ANIC/MEA的培养和测量方法的优势，并建议他们将是有用的调查长期的可塑性取决于网络拓扑结构和大小。@2007 Elsevier Inc. All rights reserved.
One of the best approaches to understanding the mechanism of information acquisition and storage is to characterize the plasticity of network activity by monitoring and stimulating individual neurons in a topologically defined network and doing this for extended periods of time. We therefore previously developed an on-chip multi-electrode array (MEA) system combined with an array of agarose microchambers (AM,Cs). It is possible to record the firing at multiple cells simultaneously for long term and topographically control the cells position and their connections. In our present study, we demonstrated the effect of tetanic stimulation in a linearly lined-up patterned network on the AMC/MEA chip. We detected reproducible activity changes that were induced by tetanic stimulation and saw that these changes were maintained for 6-24 h. The results show the advantage of our ANIC/MEA cultivation and measurements methods and suggest they will be useful for investigating the long-term plasticity depending on network topology and size. @ 2007 Elsevier Inc. All rights reserved.